AIMS Recovery after peripheral nerve injury (PNI) is often difficult, and there is no optimal treatment. Schwann cells (SCs) are important for peripheral nerve regeneration, so SC-targeting treatments have gained importance. Adipose-derived stem cells (ADSCs) and their exosomes can promote peripheral nerve repair, but their interactions with SCs are unclear. METHODS Purified SCs from sciatic nerve injury sites were harvested, and apoptosis and proliferation of SCs at post-PNI 24 hours were analyzed. The effects of coculture with ADSCs and different concentrations of ADSC-derived exosomes (ADSC-Exo) were studied through in vitro experiments by flow cytometry, CCK8 assay, immunofluorescence staining, and histological analysis. The expression of the apoptosis-related genes Bcl-2 and Bax was also analyzed by qRT-PCR. RESULTS ADSC-Exo reduced the apoptosis of SCs after PNI by upregulating the anti-apoptotic Bcl-2 mRNA expression and downregulating the pro-apoptotic Bax mRNA expression. Further, it also improved the proliferation rate of SCs. This effect was confirmed by the morphological and histological findings in PNI model rats. CONCLUSION Our results present a novel exosome-mediated mechanism for ADSC-SC cross talk that reduces the apoptosis and promotes the proliferation of SCs and may have therapeutic potential in the future.

中文翻译：

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脂肪干细胞外泌体对周围神经损伤雪旺细胞凋亡的影响[J].

周围神经损伤 (PNI) 后的 AIMS 恢复通常很困难，并且没有最佳治疗方法。雪旺细胞 (SC) 对周围神经再生很重要，因此 SC 靶向治疗变得越来越重要。脂肪干细胞 (ADSCs) 及其外泌体可以促进周围神经修复，但它们与 SCs 的相互作用尚不清楚。方法从坐骨神经损伤部位提取纯化的SCs，分析PNI后24小时SCs的凋亡和增殖情况。通过流式细胞术、CCK8 测定、免疫荧光染色和组织学分析等体外实验研究了与 ADSCs 和不同浓度 ADSC 衍生的外泌体 (ADSC-Exo) 共培养的效果。还通过qRT-PCR分析了凋亡相关基因Bcl-2和Bax的表达。结果ADSC-Exo通过上调抗凋亡Bcl-2 mRNA表达和下调促凋亡Bax mRNA表达来减少PNI后SCs的凋亡。此外，它还提高了 SCs 的增殖率。PNI 模型大鼠的形态学和组织学发现证实了这种作用。结论 我们的研究结果提出了一种新的外泌体介导的 ADSC-SC 串扰机制，可减少细胞凋亡并促进 SCs 的增殖，并可能在未来具有治疗潜力。