Background

The use of Sample-to-answer (STA) platforms for the detection of influenza A/B and respiratory syncytial virus (RSV) have greatly improved patient care. These diagnostic assays based on nucleic acid amplification are rapid, accurate and relatively easy to perform.

Objectives

We compared four such platforms for detecting FluA, FluB, and RSV from adult respiratory specimens: Hologic Panther Fusion® Flu A/B/RSV (Fusion), Cobas® Influenza A/B & RSV (Liat), Luminex Aries® Flu A/B & RSV (Aries), and Diasorin SimplexaTM Flu A/B & RSV (Simplexa).

Study Design

Nasopharyngeal (NP) swabs (n = 224) from adults were tested on these platforms and results were compared to Center for Disease Control and Prevention recommended real-time RT-PCR assay for influenza A/B and RSV. Subtyping for FluA and FluB was performed for discrepant analysis where applicable.

Results

Of the 82 FluA, 26 FluB, 15 RSV-positive specimens tested, the positive and negative percentage agreements (PPA and NPA respectively) for FluA detection were 100/100 (Fusion), 95.1/100 (Liat), 92.5/100 (Aries), and 84.1/99.3 (Simplexa); PPA and NPA for FluB detection were 92.3/99.5 (Fusion), 96/99.5 (Liat), 100/99.5 (Aries), and 80.8/100 (Simplexa); and for RSV detection were 100/100 (Fusion), 100/100 (Liat), 88.6/99.5 (Aries), and 73.3/100 (Simplexa). 82 confirmed FluA included 23 pH1N1 and 57 H3N2 strains with 2 strains remaining untyped. Of the 26 confirmed FluB, 25 were of the Yamagata lineage and 1 of unknown lineage.

Conclusion

Only 2 STA platforms demonstrated >95% PPA for the detection of all three targets while all the 4 platforms demonstrated >95% NPA for FluA, FluB and RSV.

中文翻译：

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使用成人呼吸道标本进行的四种应答A / B流感样本和RSV核酸扩增试验的比较分析。

背景

使用“应答样本”（STA）平台检测甲型/乙型流感和呼吸道合胞病毒（RSV），极大地改善了患者护理。这些基于核酸扩增的诊断测定是快速，准确并且相对容易进行的。

目标

我们比较了四种从成人呼吸道样本中检测FluA，FluB和RSV的平台：Hologic PantherFusion®流感A / B / RSV（融合），Cobas®流感A / B和RSV（利亚特），LuminexAries®流感A / B＆RSV（白羊座）和Diasorin SimplexaTM Flu A / B＆RSV（Simplexa）。

学习规划

在这些平台上对成人鼻咽（NP）拭子（n = 224）进行了测试，并将结果与​​美国疾病控制与预防中心推荐的实时RT-PCR检测方法进行了A / B和RSV流感的比较。在适用的情况下，对FluA和FluB进行了亚型分析，以进行差异分析。

结果

在82个FluA，26个FluB，15个RSV阳性标本中，用于FluA检测的阳性和阴性百分比协议（分别为PPA和NPA）分别为100/100（融合），95.1 / 100（利亚特），92.5 / 100（白羊座） ）和84.1 / 99.3（Simplexa）；用于FluB检测的PPA和NPA为92.3 / 99.5（融合），96 / 99.5（利亚特），100 / 99.5（白羊座）和80.8 / 100（Simplexa）；对于RSV检测，分别为100/100（融合），100/100（利亚特），88.6 / 99.5（白羊座）和73.3 / 100（Simplexa）。82例确诊的FluA包括23株pH1N1和57株H3N2菌株，其中2株未分型。在确认的26种FluB中，有25种属于Yamagata谱系，而1种是未知谱系。

结论

只有两个STA平台对所有三个目标的检测均显示> 95％的PPA，而所有4个平台对FluA，FluB和RSV均显示> 95％的NPA。