Functional analysis of the mitochondrial gene mitofilin in the filamentous entomopathogenic fungus Beauveria bassiana.,Fungal Genetics and Biology

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Functional analysis of the mitochondrial gene mitofilin in the filamentous entomopathogenic fungus Beauveria bassiana.
Fungal Genetics and Biology ( IF 2.4 ) Pub Date : 2019-07-04 , DOI: 10.1016/j.fgb.2019.103250
Jia-Jia Wang ₁ , Yue-Jin Peng ₁ , Ming-Guang Feng ₁ , Sheng-Hua Ying ₁

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Mitofilin acts as an essential organizer that maintains the complex architecture of the mitochondrial inner membrane (IM). In the present study, a yeast ortholog of mitofilin was characterized in the filamentous entomopathogenic fungus Beauveria bassiana; hence, it was named BbMtf. Mitochondrial localization was observed for B. bassiana mitofilin, and loss of this protein altered both the overall morphology and crista junction of the mitochondrial IM. Disruption of BbMtf resulted in reduced ATP synthesis and germination on the oligotrophic surface compared to the control. The ΔBbMtf mutant did not display significant variation in mycelial growth and stress tolerance. However, the BbMtf gene was required for conidiation and blastospore formation, and its absence led to a significant reduction in conidiation (40%) and blastospore yield (70%) in the mutant strain compared to the control. In addition, the development of the ΔBbMtf mutant in the host hemocoel was also significantly impaired, with a reduction of approximately 80% in spore concentration. Finally, disruption of BbMtf significantly attenuated fungal pathogenicity against insect hosts. Mitofilin, therefore, maintains the function of the mitochondrial IM, which contributes to the development and virulence of B. bassiana as a biocontrol fungus.

中文翻译：

丝状致病真菌球孢白僵菌中线粒体基因米托菲林的功能分析。

线粒体蛋白是维持线粒体内膜（IM）复杂结构的重要组织者。在本研究中，米托菲林的酵母直系同源物在丝状虫病真菌球孢白僵菌中得到了表征。因此，它被命名为BbMtf。观察到球孢白僵菌线粒体的线粒体定位，并且该蛋白质的损失改变了线粒体IM的整体形态和cr连接。与对照相比，BbMtf的破坏导致寡营养表面上的ATP合成和发芽减少。ΔBbMtf突变体在菌丝体生长和胁迫耐受性方面未显示出显着变化。但是，分生孢子和胚芽孢形成需要BbMtf基因，与对照相比，它的缺失导致突变菌株的分生孢子（40％）和芽孢孢子产率（70％）显着降低。此外，宿主血细胞中的ΔBbMtf突变体的发育也受到严重损害，孢子浓度降低了约80％。最后，BbMtf的破坏显着减弱了对昆虫宿主的真菌致病性。因此，线粒体蛋白保持线粒体IM的功能，线粒体IM作为一种生物防治真菌，有助于球孢杆菌的发育和致病性。BbMtf的破坏显着减弱了对昆虫宿主的真菌致病性。因此，线粒体蛋白保持线粒体IM的功能，线粒体IM作为一种生物防治真菌，有助于球孢杆菌的发育和致病性。BbMtf的破坏显着减弱了对昆虫宿主的真菌致病性。因此，线粒体蛋白保持线粒体IM的功能，线粒体IM作为一种生物防治真菌，有助于球孢杆菌的发育和致病性。

更新日期：2019-07-04

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