Temperature-responsive polymer grafted tissue culture dishes release cells as confluent living sheets in response to small changes in temperature, with recovered cell sheets retaining cell–cell communications, functional extracellular matrices and tissue-like behaviors. These features promote tissue regeneration and improve transplantation efficacy in various tissues including cartilage, heart, kidney, liver, endometrium, cornea, middle ear, periodontium, and esophageal living sheet transplants. However, the functional effects of cell sheets for salivary gland regeneration to treat hyposalivation have not yet been studied. Thus, the present study aims to both establish the viability of thermoresponsive cell sheets for use in salivary glands and then explore the delivery option (i.e., single vs. multiple layers) that would result in the most complete tissue growth in terms of cell differentiation and recovered tissue integrity. Results indicate that single cell sheets form polarized structures that maintain cell–cell junctions and secretory granules in vitro while layering of two-single cell sheets forms a glandular-like pattern in vitro. Moreover, double layer cell sheets enhance tissue formation, cell differentiation and saliva secretion in vivo. In contrast, single cell sheets demonstrated only modest gains relative to the robust growth seen with the double layer variety. Together, these data verify the utility of thermoresponsive cell sheets for use in salivary glands and indicates the double layer form to provide the best option in terms of cell differentiation and recovered tissue integrity, thereby offering a potential new therapeutic strategy for treating hyposalivation.

温度响应性聚合物接枝的组织培养皿在温度变化很小的情况下以融合的活板形式释放细胞，回收的细胞板保持细胞间的通讯，功能性细胞外基质和组织样行为。这些特征促进组织再生并提高在各种组织中的移植功效，包括软骨，心脏，肾脏，肝脏，子宫内膜，角膜，中耳，牙周膜和食道活体片移植。然而，尚未研究细胞片对唾液腺再生以治疗唾液分泌不足的功能作用。因此，本研究旨在确定用于唾液腺的热敏细胞片的生存能力，然后探讨其递送选择（即单用还是单用）。多层），就细胞分化和恢复的组织完整性而言，将导致最完整的组织生长。结果表明，单细胞片形成极化的结构，在体外保持细胞间连接和分泌颗粒，而两层单细胞片的分层在体外形成腺样的模式。而且，双层细胞片增强了体内的组织形成，细胞分化和唾液分泌。相比之下，相对于双层品种所看到的强劲增长，单电池片仅表现出适度的增长。这些数据一起证明了热敏细胞片在唾液腺中的实用性，并表明双层形式可提供细胞分化和恢复的组织完整性方面的最佳选择，