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Mesenchymal stem cell therapy improves erectile dysfunction in experimental spinal cord injury.
International Journal of Impotence Research ( IF 2.8 ) Pub Date : 2019-07-04 , DOI: 10.1038/s41443-019-0168-1 Ömercan Albayrak 1 , Tarık Emre Şener 2 , Mehmet Erşahin 3 , Suna Özbaş-Turan 4 , Ceyda Ekentok 4 , Hasan Hüseyin Tavukçu 5 , Özge Çevik 6 , Şule Çetinel 7 , Büşra Ertaş 1 , Göksel Şener 1
International Journal of Impotence Research ( IF 2.8 ) Pub Date : 2019-07-04 , DOI: 10.1038/s41443-019-0168-1 Ömercan Albayrak 1 , Tarık Emre Şener 2 , Mehmet Erşahin 3 , Suna Özbaş-Turan 4 , Ceyda Ekentok 4 , Hasan Hüseyin Tavukçu 5 , Özge Çevik 6 , Şule Çetinel 7 , Büşra Ertaş 1 , Göksel Şener 1
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The aim of this study is to investigate the therapeutic potential of adipose-derived mesenchymal stem cell (AD-MSC) from brown adipose tissue on erectile dysfunction (ED) in experimentally induced spinal cord injury in rats. 24 male Wistar rats were divided into 3 groups; control, spinal cord injury (SCI) + vehicle, and SCI + AD-MSC. To induce SCI, a standard weight-drop method that induced a moderate to severe injury (100 g/cm force) at T7-T10, was used. AD-MSC (3 × 105 cells /5 μL) was applied by local transplantation into the region of injury. At the end of four-weeks, rats underwent neurological examinations and then intracavernosal and mean arterial pressures (ICP and MAP) measurements. After decapitation, spinal cord and cavernosal tissue samples were taken to analyze neuronal nitric oxide synthase (n-NOS), proto-oncogene protein c-FOS and nerve growth factor (NGF). Tissues were also examined histologically. Spinal cord injury caused decrease on NGF and n-NOS levels while c-FOS was increased. The ICP/MAP value in vehicle-treated SCI rats was found to be significantly higher than the control group. On the other hand, in SCI + AD-MSC group, all these parameters were reversed back to control levels. AD-MSC therapy may be beneficial against erectile dysfunction in experimentally induced SCI by ameliorating neuronal damage.
中文翻译：
间充质干细胞疗法可改善实验性脊髓损伤中的勃起功能障碍。
这项研究的目的是研究来自褐色脂肪组织的脂肪间充质干细胞（AD-MSC）对大鼠实验性脊髓损伤中勃起功能障碍（ED）的治疗潜力。将24只雄性Wistar大鼠分为3组；每组3只。控制，脊髓损伤（SCI）+媒介物和SCI + AD-MSC。为了诱导SCI，使用了一种标准的减肥方法，该方法在T7-T10时引起了中度至重度伤害（100 g / cm力）。通过局部移植将AD-MSC（3×105细胞/ 5μL）应用于损伤区域。在四周结束时，对大鼠进行神经系统检查，然后进行海绵体内和平均动脉压（ICP和MAP）测量。断头后，抽取脊髓和海绵体组织样本以分析神经元一氧化氮合酶（n-NOS），原癌基因蛋白c-FOS和神经生长因子（NGF）。还对组织进行组织学检查。脊髓损伤导致NGF和n-NOS水平降低，而c-FOS升高。发现用媒介物处理的SCI大鼠中的ICP / MAP值显着高于对照组。另一方面，在SCI + AD-MSC组中，所有这些参数都被反转回控制水平。通过改善神经元损伤，AD-MSC治疗可能有益于实验诱导的SCI中的勃起功能障碍。所有这些参数都被调回控制水平。通过改善神经元损伤，AD-MSC治疗可能有益于实验诱导的SCI中的勃起功能障碍。所有这些参数都被调回控制水平。通过改善神经元损伤，AD-MSC治疗可能有益于实验诱导的SCI中的勃起功能障碍。
更新日期：2019-11-18
中文翻译：
间充质干细胞疗法可改善实验性脊髓损伤中的勃起功能障碍。
这项研究的目的是研究来自褐色脂肪组织的脂肪间充质干细胞（AD-MSC）对大鼠实验性脊髓损伤中勃起功能障碍（ED）的治疗潜力。将24只雄性Wistar大鼠分为3组；每组3只。控制，脊髓损伤（SCI）+媒介物和SCI + AD-MSC。为了诱导SCI，使用了一种标准的减肥方法，该方法在T7-T10时引起了中度至重度伤害（100 g / cm力）。通过局部移植将AD-MSC（3×105细胞/ 5μL）应用于损伤区域。在四周结束时，对大鼠进行神经系统检查，然后进行海绵体内和平均动脉压（ICP和MAP）测量。断头后，抽取脊髓和海绵体组织样本以分析神经元一氧化氮合酶（n-NOS），原癌基因蛋白c-FOS和神经生长因子（NGF）。还对组织进行组织学检查。脊髓损伤导致NGF和n-NOS水平降低，而c-FOS升高。发现用媒介物处理的SCI大鼠中的ICP / MAP值显着高于对照组。另一方面，在SCI + AD-MSC组中，所有这些参数都被反转回控制水平。通过改善神经元损伤，AD-MSC治疗可能有益于实验诱导的SCI中的勃起功能障碍。所有这些参数都被调回控制水平。通过改善神经元损伤，AD-MSC治疗可能有益于实验诱导的SCI中的勃起功能障碍。所有这些参数都被调回控制水平。通过改善神经元损伤，AD-MSC治疗可能有益于实验诱导的SCI中的勃起功能障碍。
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