Aging has been established as a major risk factor for prevalent diseases and hence, the development of anti-aging medicines is of great importance. Recently, herbal fermented beverages have emerged as a promising source of potential anti-aging drug. Pru, a traditional Cuban refreshment produced by decoction and fermentation of multispecies plants with sugar, has been consumed for many years and is claimed to have multiple medicinal properties. Besides the traditional method, Pru is also manufactured industrially. The present study analyzed the major components of both traditional Pru (TP) and industrial Pru (IP) to reveal their potential application in promoting the health span. We performed desorption electrospray ionization-mass spectrometry (DESI-MS) and acquired mass spectra by scanning over the 50-1200 m/z range in both positive and negative ion modes. Fourier transform ion cyclotron resonance (FTICR) tandem mass spectrometry (MS/MS) was performed for validating the compound assignments. Three important compounds were identified by comparing the MS and MS/MS spectra with reported literature and the online database. One of the identified compounds, gluconic acid, was found to be the most abundant shared metabolite between TP and IP whereas the other two compounds, magnoflorine and levan were exclusively detected in TP. The present study is the first report of component profiling in Cuban traditional and industrial Pru using DESI-MS and FTICR MS/MS, and reveals the potential application of Pru as a health-promoting agent.

中文翻译：

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通过解吸电喷雾电离质谱和 FTICR 串联质谱分析古巴传统茶点 Pru 的潜在抗衰老饮料

衰老已被确定为流行疾病的主要危险因素，因此，抗衰老药物的开发非常重要。最近，草本发酵饮料已成为潜在的抗衰老药物的有希望的来源。Pru 是一种传统的古巴点心，由多种植物加糖煎煮发酵而成，已被食用多年，据称具有多种药用价值。除了传统的方法，Pru 也是工业化生产的。本研究分析了传统 Pru (TP) 和工业 Pru (IP) 的主要成分，以揭示它们在促进健康跨度方面的潜在应用。我们进行了解吸电喷雾电离质谱 (DESI-MS)，并通过在正离子和负离子模式下扫描 50-1200 m/z 范围来获得质谱。执行傅里叶变换离子回旋共振 (FTICR) 串联质谱 (MS/MS) 以验证化合物分配。通过将 MS 和 MS/MS 谱图与已报道的文献和在线数据库进行比较，鉴定出三种重要的化合物。其中一种已鉴定的化合物葡萄糖酸被发现是 TP 和 IP 之间最丰富的共享代谢物，而其他两种化合物 Magnoflorine 和果聚糖仅在 TP 中检测到。本研究是使用 DESI-MS 和 FTICR MS/MS 对古巴传统和工业 Pru 进行成分分析的第一份报告，