The first part of this review article lists examples of complete, empirical de novo design that made important contributions to the development of the field and initiated challenging projects. The second part of this article deals with computational design of novel enzymes in native protein scaffolds; active designs were refined through random and site-directed mutagenesis producing artificial enzymes with nearly native enzyme- like activities against a number of non-natural substrates. Combining aspects of de novo design and biological evolution of nature’s enzymes has started and will accelerate the development of novel enzyme activities.

中文翻译：

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催化多肽和蛋白质的设计

这篇评论文章的第一部分列出了完整的，从头开始的经验设计实例，这些实例为该领域的发展做出了重要贡献，并发起了具有挑战性的项目。本文的第二部分讨论了天然蛋白质支架中新型酶的计算设计。通过随机和定点诱变，对活性设计进行改进，产生对多种非天然底物具有接近天然酶样活性的人工酶。从头设计与自然界酶的生物进化相结合的方法已经开始，并将加速新型酶活性的发展。