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Simultaneous determination of cardiovascular drugs in dried blood spot by liquid chromatography-tandem mass spectrometry
Journal of Food and Drug Analysis ( IF 2.6 ) Pub Date : 2019-10-01 , DOI: 10.1016/j.jfda.2019.06.001 Hyung Min Kim 1 , Ju-Hwan Park 1 , Nguyen Phuoc Long 1 , Dae-Duk Kim 2 , Sung Won Kwon 2
Journal of Food and Drug Analysis ( IF 2.6 ) Pub Date : 2019-10-01 , DOI: 10.1016/j.jfda.2019.06.001 Hyung Min Kim 1 , Ju-Hwan Park 1 , Nguyen Phuoc Long 1 , Dae-Duk Kim 2 , Sung Won Kwon 2
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A dried blood spot (DBS) sampling method was exploited to extract cardiovascular drugs using a small volume of whole blood of human and rodent. Thereafter, an analytical method using liquid chromatography with tandem mass spectrometry (LC-MS/MS) was developed and validated for the determination of 12 cardiovascular drugs. A 6 mm internal diameter disc containing 10 μL of blood was punched from a specifically designed card and analyzed by LC-MS/MS using a gradient elution method with a total run time of 16 min. For sample separation, a universal octadecyl-silica column was used with a flow rate of 0.2 mL/min. The developed method was validated in terms of linearity, accuracy, and precision, which showed satisfactory results. In addition, the matrix effects were closely investigated to confirm the extraction efficiency. Additionally, the stability was tested by storing DBSs at room temperature; the results showed that these drugs were stable for at least 30 days. Accordingly, the proposed LC-MS/MS method is capable to analyze several cardiovascular drugs in a single analysis. It can be applied to therapeutic drug monitoring in patients as well as in the in vivo settings.
中文翻译：
液相色谱-串联质谱法同时测定干血斑中心血管药物
利用干血斑 (DBS) 采样方法，使用少量人类和啮齿动物的全血提取心血管药物。此后，开发并验证了一种使用液相色谱和串联质谱 (LC-MS/MS) 的分析方法，用于测定 12 种心血管药物。从专门设计的卡片上冲出一个内径为 6 mm 的圆盘，其中包含 10 μL 血液，并使用梯度洗脱方法通过 LC-MS/MS 进行分析，总运行时间为 16 分钟。对于样品分离，使用流速为 0.2 mL/min 的通用十八烷基硅胶柱。所开发的方法在线性、准确度和精密度方面进行了验证，结果令人满意。此外，对基质效应进行了深入研究，以确认提取效率。此外，通过在室温下储存 DBS 来测试稳定性；结果表明，这些药物至少可以稳定 30 天。因此，所提出的 LC-MS/MS 方法能够在一次分析中分析几种心血管药物。它可以应用于患者以及体内环境中的治疗药物监测。
更新日期：2019-10-01
中文翻译：
液相色谱-串联质谱法同时测定干血斑中心血管药物
利用干血斑 (DBS) 采样方法，使用少量人类和啮齿动物的全血提取心血管药物。此后，开发并验证了一种使用液相色谱和串联质谱 (LC-MS/MS) 的分析方法，用于测定 12 种心血管药物。从专门设计的卡片上冲出一个内径为 6 mm 的圆盘，其中包含 10 μL 血液，并使用梯度洗脱方法通过 LC-MS/MS 进行分析，总运行时间为 16 分钟。对于样品分离，使用流速为 0.2 mL/min 的通用十八烷基硅胶柱。所开发的方法在线性、准确度和精密度方面进行了验证，结果令人满意。此外，对基质效应进行了深入研究，以确认提取效率。此外，通过在室温下储存 DBS 来测试稳定性；结果表明，这些药物至少可以稳定 30 天。因此，所提出的 LC-MS/MS 方法能够在一次分析中分析几种心血管药物。它可以应用于患者以及体内环境中的治疗药物监测。
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