Systematically characterize the substance basis of Jinzhen oral liquid and their pharmacological mechanism using UPLC-Q-TOF/MS combined with network pharmacology analysis,Journal of Food and Drug Analysis

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Systematically characterize the substance basis of Jinzhen oral liquid and their pharmacological mechanism using UPLC-Q-TOF/MS combined with network pharmacology analysis
Journal of Food and Drug Analysis ( IF 2.6 ) Pub Date : 2019-07-01 , DOI: 10.1016/j.jfda.2019.05.007
Jing-Yan Guo ₁ , Dong-Mei Wang ₁ , Meng-Jiao Wang ₁ , Jun Zhou ₂ , Ying-Ni Pan ₁ , Zheng-Zhong Wang ₂ , Wei Xiao ₃ , Xiao-Qiu Liu ₁

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Jinzhen oral liquid (JZ) is a classical traditional Chinese medicine formula used for the treatment of children lung disease. However, the effective substance of JZ is still unclear. In this study, we used lung injury rat model to study the protective effect of JZ, through UPLC-Q-TOF/MS detection coupled with metabolic research and network pharmacology analysis. Fortunately, 31 absorbed prototype constituents and 41 metabolites were identified or tentatively characterized based on UPLC-Q-TOF/MS analysis, and the possible metabolic pathways were hydroxylation, sulfation and glucuronidation. We optimized the data screening in the early stage of network pharmacology by collecting targets based on adsorbed constituents, and further analyzed the main biological processes and pathways. 24 selected core targets were frequently involved in reactive oxygen species metabolic process, dopaminergic synapse pathway and so on, which might play important roles in the mechanisms of JZ for the treatment of lung injury. Overall, the absorbed constituents and their possible metabolic pathways, as well as the absorbed constituent-target-disease network provided insights into the mechanisms of JZ for the treatment of lung injury. Further studies are needed to validate the biological processes and effect pathways of JZ.

中文翻译：

UPLC-Q-TOF/MS结合网络药理分析系统表征金珍口服液的物质基础及其药理作用机制

金珍口服液（JZ）是一种用于治疗小儿肺病的经典中药配方。然而，JZ的有效物质仍不清楚。本研究采用肺损伤大鼠模型，通过UPLC-Q-TOF/MS检测结合代谢研究和网络药理学分析，研究JZ的保护作用。幸运的是，基于 UPLC-Q-TOF/MS 分析鉴定或初步表征了 31 种吸收的原型成分和 41 种代谢物，可能的代谢途径是羟基化、硫酸化和葡萄糖醛酸化。我们通过基于吸附成分收集目标优化了网络药理学早期的数据筛选，并进一步分析了主要的生物学过程和途径。24个选定的核心靶点经常参与活性氧代谢过程、多巴胺能突触通路等，可能在JZ治疗肺损伤的机制中发挥重要作用。总体而言，吸收的成分及其可能的代谢途径，以及吸收的成分-靶标-疾病网络，为了解 JZ 治疗肺损伤的机制提供了见解。需要进一步的研究来验证JZ的生物学过程和作用途径。以及吸收的成分-靶标-疾病网络为JZ治疗肺损伤的机制提供了见解。需要进一步的研究来验证JZ的生物学过程和作用途径。以及吸收的成分-靶标-疾病网络为JZ治疗肺损伤的机制提供了见解。需要进一步的研究来验证JZ的生物学过程和作用途径。

更新日期：2019-07-01

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