Integrative structure modeling computationally combines data from multiple sources of information with the aim of obtaining structural insights that are not revealed by any single approach alone. In the first part of this review, we survey the commonly used sources of structural information and the computational aspects of model building. Throughout the past decade, integrative modeling was applied to various biological systems, with a focus on large protein complexes. Recent progress in the field of cryo-electron microscopy (cryo-EM) has resolved many of these complexes to near-atomic resolution. In the second part of this review, we compare a range of published integrative models with their higher-resolution counterparts with the aim of critically assessing their accuracy. This comparison gives a favorable view of integrative modeling and demonstrates its ability to yield accurate and informative results. We discuss possible roles of integrative modeling in the new era of cryo-EM and highlight future challenges and directions.

中文翻译：

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集成结构建模：概述和评估。

集成结构建模以计算方式组合了来自多个信息源的数据，目的是获得单独的任何一种方法都无法揭示的结构见解。在本文的第一部分中，我们调查了结构信息的常用来源以及模型构建的计算方面。在过去的十年中，集成建模已应用于各种生物系统，重点是大型蛋白质复合物。冷冻电子显微镜（cryo-EM）领域的最新进展已将许多这些复合物解析为接近原子的分辨率。在本评论的第二部分中，我们比较了一系列已发布的集成模型及其分辨率更高的模型，目的是严格评估其准确性。这种比较为集成建模提供了一个有利的视角，并展示了其产生准确而有益的结果的能力。我们讨论了集成建模在cryo-EM新时代中可能扮演的角色，并重点介绍了未来的挑战和方向。