Cold atmospheric plasma (CAP) is an emerging source for the locally defined delivery of reactive species, and its clinical potential has been identified in the control of inflammatory processes, such as acute and chronic wounds, or cancerous lesions. Lipids, due to their localization and chemical structure as ideal targets for oxidative species, are relevant modifiers of physiological processes. Human forehead lipids collected on a target were treated by an argon plasma jet and immediately analyzed by direct-infusion high-resolution tandem mass spectrometry (DI-MS2) or liquid chromatography-tandem MS (RP-LC/MS2). Subsequent data analysis was performed by LipidHunter (University of Leipzig), LipidXplorer (Max Planck Institute of Molecular Cell Biology and Genetics, Dresden), and LipidSearch (Thermo Scientific). With either MS method, all major lipid classes of sebum lipids were detected. Significant differences regarding triacylglycerols (predominantly identified in RP-LC/MS2) and ceramides (predominantly identified in DI-MS2) indicate experimental- or approach-inherent distinctions. A CAP-driven oxidation of triacyclglycerols, ceramides, and cholesteryl esters was detected such as truncations and hydroperoxylations, but at a significantly lower extent than expected. Scavenging of reactive species due to naturally present antioxidants in the samples and the absence of a liquid interphase to allow reactive species deposition by the CAP will have contributed to the limited amount of oxidation products observed. In addition, limitations of the software's capability of identifying unexpected oxidized lipids potentially led to an underestimation of the CAP impact on skin lipids, indicating a need for further software development. With respect to the clinical application of CAP, the result indicates that intact skin with its sebum/epidermal lipid overlay is well protected and that moderate treatment will yield limited (if any) functional consequences in the dermal tissue.

中文翻译：

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冷空气血浆（CAP）对皮肤脂质的氧化修饰：使用RP-LC / MS2和DI-ESI / MS2的标准化方法。

大气冷血浆（CAP）是用于局部定义反应性物质递送的新兴来源，其临床潜力已在控制炎症过程（如急性和慢性伤口或癌性病变）中得到了证实。由于脂质的定位和化学结构，它们是氧化物质的理想靶标，因此它们是生理过程的相关修饰剂。用氩等离子体喷射器处理收集在靶标上的人额脂质，并立即通过直接输注高分辨率串联质谱法（DI-MS2）或液相色谱-串联质谱仪（RP-LC / MS2）进行分析。随后的数据分析由LipidHunter（莱比锡大学），LipidXplorer（马克斯·普朗克分子细胞生物学和遗传学研究所，德累斯顿）和LipidSearch（Thermo Scientific）进行。无论采用哪种MS方法，检出了皮脂的所有主要脂质类别。关于三酰基甘油（主要在RP-LC / MS2中鉴定）和神经酰胺（主要在DI-MS2中鉴定）之间的显着差异表明了实验或方法固有的区别。可以检测到由CAP驱动的三环甘油，神经酰胺和胆固醇酯的氧化反应，例如截短和加氢过氧化反应，但氧化程度远低于预期。由于样品中天然存在的抗氧化剂而导致的清除反应性物种，以及由于缺少液相相而使CAP无法沉积反应性物种，这将导致观察到的氧化产物数量有限。此外，该软件的局限性在于 识别意外氧化脂质的能力可能导致低估CAP对皮肤脂质的影响，这表明需要进一步的软件开发。关于CAP的临床应用，结果表明，皮脂/表皮脂质覆盖物完整的皮肤得到了很好的保护，适度的治疗将在皮肤组织中产生有限的（如果有的话）功能性后果。