During a “capping ceremony”, the RNA snake obtains one of three distinct 5′‐caps, changing its identity to either snRNA or mRNA. In our work, we have combined chemical and enzymatic synthesis of RNA with site‐specific methyltransferases (MTases) to obtain highly cap‐modified RNAs on a preparative scale. MTases hTgs and GlaTgs allow access to snRNAs that are either mono‐ or dimethylated at the N2 position of the terminal G (crowns 2m,7mGppp and 2m,2m,7mGppp, left and center), whereas CMTR1 yielded the cap1 structure (right crown), a structural motif in mRNA important for translation. More information can be found in the full paper by F. Debart. A. Rentmeister, et al. on page 1693 in Issue 13, 2019 (DOI: 10.1002/cbic.201900037). Photo of Montpellier Cathedral by Wolfgang Staudt, protected under a Creative Commons License. Copyright for the image: Nina Knubel and Dr. Daniela Stummer.

中文翻译：

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封面：将化学合成与酶法甲基化相结合以使用带有各种5'帽的短RNA（ChemBioChem 13/2019）

在“封顶仪式”中，RNA蛇获得了三个不同的5'帽之一，将其身份更改为snRNA或mRNA。在我们的工作中，我们将RNA的化学和酶促合成与位点特异性甲基转移酶（MTase）结合在一起，以制备规模获得高度帽修饰的RNA。MTases hTgs和GlaTgs允许访问在末端G的N2位置（冠2m，7mGppp和2m，2m，7mGppp，左侧和中央）的N2位被单或二甲基化的snRNA，而CMTR1产生了cap1结构（右冠） ，mRNA中对翻译重要的结构性基序。有关更多信息，请参见F. Debart的全文。A.Rentmeister等。就在第13期，2019页1693（DOI：10.1002 / cbic.201900037）。沃尔夫冈·施陶特（Wolfgang Staudt）的蒙彼利埃大教堂照片，受创用CC许可保护。图片版权：Nina Knubel和Daniela Stummer博士。