Electrochemiluminescence (ECL) is a widely used analytical technique with the advantages of high sensitivity and low background signal. The recent and rapid development of electrochemical materials, luminophores, and optical elements significantly increases the ECL signals and, thus, ECL imaging with enhanced spatial and temporal resolutions is realized. Currently, ECL imaging is successfully applied to high-throughput bioanalysis and to visualize the distribution of molecules at single cells. Compared with other optical bioassays, no optical excitation is involved in imaging, so the approach avoids a background signal from illumination and increases the detection sensitivity. This review highlights some of the most exciting developments in this field, including the mechanisms, electrode designs, and the applications of ECL imaging in bioanalysis and at single cells and particles.

中文翻译：

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用于生物分析的电化学发光成像。

电化学发光（ECL）是一种广泛使用的分析技术，具有高灵敏度和低背景信号的优点。电化学材料，发光体和光学元件的近来和快速发展显着增加了ECL信号，因此，实现了具有增强的时空分辨率的ECL成像。当前，ECL成像已成功地应用于高通量生物分析，并可视化了单细胞中分子的分布。与其他光学生物测定法相比，成像中不涉及光学激发，因此该方法避免了来自照明的背景信号并提高了检测灵敏度。这篇评论重点介绍了该领域的一些最令人振奋的进展，包括机理，电极设计，