Host-derived fecal microRNAs can indicate gut microbiota healthiness and ability to induce inflammation.,Theranostics

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Host-derived fecal microRNAs can indicate gut microbiota healthiness and ability to induce inflammation.
Theranostics ( IF 12.4 ) Pub Date : 2019-01-01 , DOI: 10.7150/thno.35282
Emilie Viennois ₁ , Benoit Chassaing _{1,

2} , Anika Tahsin ₁ , Adani Pujada ₁ , Lixin Wang _{1,

3} , Andrew T Gewirtz ₁ , Didier Merlin _{1,

3}

Affiliation

Disruption of intestine-microbiota symbiosis can result in chronic gut inflammation. We hypothesize that assessing the initial inflammatory potential of the microbiota in patients is essential and that host-derived miRNAs, which can be found in feces, could fulfill this function. We investigated whether the gut microbiota composition impacts the fecal miRNA profile and thereby indicates its ability to influence intestinal inflammation. Methods: We used high-throughput qPCR to compare fecal miRNA profile between germ-free and conventional mice. Conventionalization of germfree mice by various colitogenic and non-colitogenic microbiotas (IL10-/- and TLR5-/- associated microbiota) was performed. Results: We identified 12 fecal miRNAs impacted by the presence of a microbiota. Conventionalization of germfree mice by various colitogenic and non-colitogenic microbiotas associated with the development of intestinal inflammation (IL10-/- and TLR5-/- associated microbiota) yielded distinctively altered fecal miRNA profiles compared to that of mice receiving a "healthy" microbiota. Correlation analysis revealed the existence of interactions between the 12 abovementioned miRNAs and specific microbiota members. Conclusion: These results showed that fecal miRNA profile can be differentially and specifically impacted by microbiota composition, and that miRNA could importantly serve as markers of the colitogenic potential of the microbiota. This is particularly relevant to assess individual state of the microbiota in patients with dysbiosis-related disorders, such as IBD and potentially determine their ability to respond to therapeutics.

中文翻译：

宿主来源的粪便 microRNA 可以表明肠道微生物群的健康状况和诱发炎症的能力。

肠道微生物群共生的破坏可导致慢性肠道炎症。我们假设评估患者微生物群的初始炎症潜力是必不可少的，并且可以在粪便中发现的宿主衍生的 miRNA 可以实现这一功能。我们研究了肠道微生物群组成是否影响粪便 miRNA 谱，从而表明其影响肠道炎症的能力。方法：我们使用高通量 qPCR 来比较无菌小鼠和常规小鼠之间的粪便 miRNA 谱。通过各种致结肠炎和非结肠炎微生物群（IL10-/- 和 TLR5-/- 相关微生物群）对无菌小鼠进行常规化。结果：我们确定了 12 种受微生物群存在影响的粪便 miRNA。与接受“健康”微生物群的小鼠相比，与肠道炎症的发展相关的各种致结肠炎和非结肠炎微生物群（IL10-/- 和 TLR5-/- 相关微生物群）对无菌小鼠的常规化产生了明显改变的粪便 miRNA 谱。相关性分析揭示了上述 12 种 miRNA 与特定微生物群成员之间存在相互作用。结论：这些结果表明粪便 miRNA 谱可以受到微生物群组成的差异和特异性影响，并且 miRNA 可以重要地作为微生物群潜在结肠炎的标志物。这与评估与生态失调相关的疾病患者的微生物群的个体状态特别相关，

更新日期：2019-01-01

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