Microbes such as Pseudomonas aeruginosa are often challenged by rapidly changing nutritional environments. In order to adapt to these shifts in nutrient availability, bacteria exert tight transcriptional control over the enzymes of central metabolism. This transcriptional control is orchestrated by a series of transcriptional repressors and activators. Although a number of these transcription factors have been identified, many others remain uncharacterized. Here, we present a simple pipeline to uncover and validate the targets of uncharacterized transcriptional regulators in P. aeruginosa. We use this approach to identify and confirm that an orthologue of the Pseudomonas fluorescens transcriptional regulator (RccR) binds to the upstream region of isocitrate lyase (aceA) in P. aeruginosa, thereby repressing flux through the glyoxylate shunt during growth on non‐C2 carbon sources.

中文翻译：

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铜绿假单胞菌中央碳代谢的转录调控。

诸如铜绿假单胞菌之类的微生物通常受到营养环境快速变化的挑战。为了适应营养物可利用性的这些变化，细菌对中枢代谢酶进行了严格的转录控制。该转录控制由一系列转录阻遏物和激活物共同调控。尽管已鉴定出许多这些转录因子，但许多其他转录因子仍未表征。在这里，我们提出了一个简单的管道来发现和验证铜绿假单胞菌中未表征的转录调节因子的靶标。我们使用这种方法来识别并确认荧光假单胞菌的直系同源物转录调节因子（RccR）结合到铜绿假单胞菌的异柠檬酸裂解酶（aceA）的上游区域，从而在非C2碳源上生长期间抑制通过乙醛酸分流器的通量。