Significant efforts are necessary to introduce new dietary protein sources to feed a growing world population while maintaining food supply chain sustainability. Such a sustainable protein transition includes the use of highly modified proteins from side streams or the introduction of new protein sources that may lead to increased clinically relevant allergic sensitization. With food allergy being a major health problem of increasing concern, understanding the potential allergenicity of new or modified proteins is crucial to ensure public health protection. The best predictive risk assessment methods currently relied on are in vivo models, making the choice of endpoint parameters a key element in evaluating the sensitizing capacity of novel proteins. Here, we provide a comprehensive overview of the most frequently used in vivo and ex vivo endpoints in murine food allergy models, addressing their strengths and limitations for assessing sensitization risks. For optimal laboratory‐to‐laboratory reproducibility and reliable use of predictive tests for protein risk assessment, it is important that researchers maintain and apply the same relevant parameters and procedures. Thus, there is an urgent need for a consensus on key food allergy parameters to be applied in future food allergy research in synergy between both knowledge institutes and clinicians.

中文翻译：

---

小鼠食物过敏模型的体内和体外终点概述：适合评估新型蛋白质的致敏能力？

需要做出重大努力来引入新的膳食蛋白质来源，以养活不断增长的世界人口，同时保持食品供应链的可持续性。这种可持续的蛋白质转变包括使用来自侧流的高度修饰的蛋白质或引入新的蛋白质来源，这可能会导致增加临床相关的过敏性致敏。由于食物过敏是一个日益受到关注的主要健康问题，因此了解新蛋白质或修饰蛋白质的潜在过敏性对于确保公众健康保护至关重要。目前依赖的最佳预测风险评估方法是体内模型，这使得终点参数的选择成为评估新型蛋白质致敏能力的关键因素。这里，我们全面概述了鼠类食物过敏模型中最常用的体内和体外终点，探讨了它们在评估致敏风险方面的优势和局限性。为了获得最佳的实验室间重现性和蛋白质风险评估预测测试的可靠使用，研究人员保持和应用相同的相关参数和程序非常重要。因此，迫切需要就关键的食物过敏参数达成共识，以便在知识机构和临床医生之间的协同作用下应用于未来的食物过敏研究。研究人员保持和应用相同的相关参数和程序很重要。因此，迫切需要就关键的食物过敏参数达成共识，以便在知识机构和临床医生之间的协同作用下应用于未来的食物过敏研究。研究人员保持和应用相同的相关参数和程序很重要。因此，迫切需要就关键的食物过敏参数达成共识，以便在知识机构和临床医生之间的协同作用下应用于未来的食物过敏研究。