BACKGROUND The dispersal of airborne norovirus (NoV) particles from the floor after contamination with faeces or vomit is a challenge for infection control, as this pathogen is infectious at low doses. Therefore, it is imperative to establish a safe protocol for floor decontamination. AIM To assess the presence of residual NoV-GII particles on floors and airborne particles following various floor decontamination procedures. METHODS Two types of floor (vinyl and granite) were contaminated intentionally with 10% human faeces, positive for NoV-GII. Two decontamination protocols were implemented: cleaning followed by disinfection using 1% sodium hypochlorite, and cleaning followed by disinfection using a manual ultraviolet C (UV-C) light device. Swab samples were taken from the floors, and air samples were obtained using an air sampler. The TaqMan method for real-time reverse transcription-quantitative polymerase chain reaction was employed for analysis. FINDINGS The disinfection protocol using 1% sodium hypochlorite after cleaning proved to be more effective than cleaning followed by UV-C light exposure (P<0.001). Viral particles were detected in 27 of 36 air samples after cleaning, with no significant difference between the two floor types. On average, 617 genome copies/sample were identified in air samples after cleaning, but the number decreased gradually after disinfection. CONCLUSION NoV-GII can be aerosolized during floor cleaning, and its particles may be inhaled and then swallowed or can settle on surfaces. Therefore, residual viral particles on floors must be fully eliminated. Cleaning followed by 10 min of 1% sodium hypochlorite disinfection proved to be the superior decontamination protocol.

中文翻译：

---

经过多种净化方案后，诺如病毒可从地板和空气中回收。

背景技术在粪便或呕吐物污染后，从地板上散播空中诺如病毒（NoV）颗粒对于控制感染是一项挑战，因为这种病原体在低剂量下具有传染性。因此，必须建立安全的地板净化方案。目的评估地板经过各种去污程序后残留的NoV-GII颗粒和空气传播颗粒的存在。方法两种类型的地板（乙烯基地板和花岗岩地板）被10％的人类粪便故意污染，对NoV-GII呈阳性。实施了两种去污方案：清洁，然后使用1％的次氯酸钠消毒，以及清洁后使用手动紫外线C（UV-C）照明设备进行消毒。从地板上取拭子样品，并使用空气采样器获得空气样品。用TaqMan方法进行实时逆转录-定量聚合酶链反应。结果清洗后使用1％次氯酸钠的消毒方案被证明比清洗和UV-C曝光更有效（P <0.001）。清洁后，在36个空气样本中的27个中检测到病毒颗粒，两种地板类型之间无显着差异。清洗后空气样品中平均鉴定出617个基因组拷贝/样品，但消毒后数量逐渐减少。结论NoV-GII可以在地板清洁过程中雾化，并且其颗粒可能被吸入然后被吞下或沉降在表面上。因此，必须完全清除地板上残留的病毒颗粒。