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A Post-domestication Mutation, Dt2, Triggers Systemic Modification of Divergent and Convergent Pathways Modulating Multiple Agronomic Traits in Soybean.
Molecular Plant ( IF 17.1 ) Pub Date : 2019-05-29 , DOI: 10.1016/j.molp.2019.05.010
Dajian Zhang 1 , Xutong Wang 2 , Shuo Li 3 , Chaofan Wang 4 , Michael J Gosney 5 , Michael V Mickelbart 6 , Jianxin Ma 7
Affiliation  

The semi-determinate stem growth habit in leguminous crops, similar to the “green revolution” semi-dwarf trait in cereals, is a key plant architecture trait that affects several other traits determining grain yield. In soybean semi-determinacy is modulated by a post-domestication gain-of-function mutation in the gene, Dt2, which encodes an MADS-box transcription factor. However, its role in systemic modification of stem growth and other traits is unknown. In this study, we show that Dt2 functions not only as a direct repressor of Dt1, which prevents terminal flowering, but also as a direct activator of putative floral integrator/identity genes including GmSOC1, GmAP1, and GmFUL, which likely promote flowering. We also demonstrate that Dt2 functions as a direct repressor of the putative drought-responsive transcription factor gene GmDREB1D, and as a direct activator of GmSPCH and GmGRP7, which are potentially associated with asymmetric division of young epidermal cells and stomatal opening, respectively, and may affect the plant's water-use efficiency (WUE). Intriguingly, Dt2 was found to be a direct activator or repressor of the precursors of eight microRNAs targeting genes potentially associated with meristem maintenance, flowering time, stomatal density, WUE, and/or stress responses. This study thus reveals the molecular basis of pleiotropy associated with plant productivity, adaptability, and environmental resilience.



中文翻译:


驯化后突变 Dt2 触发调节大豆多种农艺性状的发散和趋同途径的系统性改变。



豆科作物的半决定性茎生长习性,类似于谷物中的“绿色革命”半矮化性状,是一个关键的植物结构性状,影响着决定谷物产量的其他几个性状。在大豆中,半决定性是通过基因Dt2中的驯化后功能获得性突变来调节的,该基因编码 MADS-box 转录因子。然而,它在茎生长和其他性状的系统改变中的作用尚不清楚。在这项研究中,我们表明 Dt2 不仅可以作为Dt1的直接阻遏物(阻止终末开花),而且可以作为假定的花整合/识别基因(包括GmSOC1GmAP1GmFUL )的直接激活剂(可能促进开花)。我们还证明,Dt2 可以作为假定的干旱反应转录因子基因GmDREB1D的直接抑制子,以及GmSPCHGmGRP7的直接激活子,它们分别与年轻表皮细胞的不对称分裂和气孔开放有关,并且可能影响工厂的水分利用效率(WUE)。有趣的是,Dt2被发现是八种microRNA前体的直接激活剂或阻遏剂,其目标基因可能与分生组织维持、开花时间、气孔密度、WUE和/或应激反应相关。因此,这项研究揭示了与植物生产力、适应性和环境恢复力相关的多效性的分子基础。

更新日期:2019-05-29
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