Assessment of the imaging quality in localisation-based super-resolution techniques relies on an accurate characterisation of the imaging setup and analysis procedures. Test samples can provide regular feedback on system performance and facilitate the implementation of new methods. While multiple test samples for regular, 2D imaging are available, they are not common for more specialised imaging modes. Here, we analyse robust test samples for 3D and quantitative super-resolution imaging, which are straightforward to use, are time- and cost-effective and do not require experience beyond basic laboratory and imaging skills. We present two options for assessment of 3D imaging quality, the use of microspheres functionalised for DNA-PAINT and a commercial DNA origami sample. A method to establish and assess a qPAINT workflow for quantitative imaging is demonstrated with a second, commercially available DNA origami sample.

中文翻译：

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使用 DNA-PAINT 和 DNA 折纸测试样本进行 3D 超分辨率显微镜性能和定量分析评估

在基于定位的超分辨率技术中对成像质量的评估依赖于成像设置和分析程序的准确表征。测试样本可以提供有关系统性能的定期反馈，并促进新方法的实施。虽然可以使用多个用于常规 2D 成像的测试样本，但它们对于更专业的成像模式并不常见。在这里，我们分析了用于 3D 和定量超分辨率成像的可靠测试样本，这些样本易于使用、节省时间和成本，并且不需要超出基本实验室和成像技能的经验。我们提供了两种评估 3D 成像质量的选项，使用功能化用于 DNA-PAINT 的微球和商业 DNA 折纸样本。