Abstract A rapid method for simultaneous determination of five synthetic edible pigments (SEPs) including tartrazine (TA), ponceau 4R (PO), sunset yellow (SY), brilliant blue (BB) and erythrosine (ER) in beverages with titania-based RP-HPLC has been developed. The good linear relationships were obtained in the concentration ranges of 2.5–40 μg mL−1 for TA, PO and SY, 0.75–12 μg mL−1 for BB, and 1.25–20 μg mL−1 for ER, respectively. The detection limits (LODs) of five SEPs were 0.042, 0.021, 0.042, 0.0005, and 0.021 μg mL−1, respectively. The average recoveries were between 92.2% and 106.3%. Relative standard deviations (RSD, n = 7) for five SEPs were less than 1.18%. The precision and accuracy of the method can meet the requirements of HPLC analysis. In addition, the thermodynamic parameters of retention of the pigments in the titania column such as enthalpy (ΔHο), entropy (ΔSο) and Gibbs free energy (ΔGο) were also determined.

中文翻译：

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二氧化钛反相高效液相色谱法同时测定饮料中的合成食用色素

摘要 采用二氧化钛基 RP 同时测定饮料中酒石黄 (TA)、丽春红 4R (PO)、日落黄 (SY)、亮蓝 (BB) 和赤藓红 (ER) 等五种合成食用色素 (SEP) 的快速方法-HPLC 已开发。在 TA、PO 和 SY 的 2.5-40 μg mL-1、BB 的 0.75-12 μg mL-1 和 ER 的 1.25-20 μg mL-1 的浓度范围内获得了良好的线性关系。五个 SEP 的检测限 (LOD) 分别为 0.042、0.021、0.042、0.0005 和 0.021 μg mL-1。平均回收率在 92.2% 和 106.3% 之间。五个 SEP 的相对标准偏差 (RSD，n = 7) 小于 1.18%。该方法的精密度和准确度能够满足HPLC分析的要求。此外，