Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.

中文翻译：

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拆分绿色荧光蛋白：范围，局限性和展望。

许多蛋白质可以被分解成片段，这些片段在没有共价键的情况下自发地重新组装成功能性蛋白质。对于分裂的绿色荧光蛋白（GFP），片段重组会导致荧光读数，这种读数已被广泛用于研究蛋白质与蛋白质之间的相互作用。我们回顾了这种方法的范围和局限性，以及拆分GFP在多功能传感器，分子胶，光遗传学工具和光物理研究平台中的其他各种应用。