Biological molecules are often highly dynamic, and this flexibility can be critical for function. The large range of sampled timescales and the fact that many of the conformers that are continually explored are only transiently formed and sparsely populated challenge current biophysical approaches. Solution nuclear magnetic resonance (NMR) spectroscopy has emerged as a powerful method for characterizing biomolecular dynamics in detail, even in cases where excursions involve short-lived states. Here, we briefly review a number of NMR experiments for studies of biomolecular dynamics on the microsecond-to-second timescale and focus on applications to protein and nucleic acid systems that clearly illustrate the functional relevance of motion in both health and disease.

中文翻译：

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健康和疾病中蛋白质动力学的NMR视图。

生物分子通常是高度动态的，这种灵活性对于功能至关重要。大范围的采样时间尺度以及不断探索的许多构象异构体仅是暂时形成且分布稀疏的事实，对当前的生物物理方法提出了挑战。溶液核磁共振（NMR）光谱已经成为一种详细表征生物分子动力学的有力方法，即使在短途旅行涉及短暂生命的情况下也是如此。在这里，我们简要回顾了许多NMR实验，以研究微秒到秒的时间范围内的生物分子动力学，并重点研究了蛋白质和核酸系统的应用，这些应用清楚地说明了运动在健康和疾病中的功能相关性。