Imaging techniques greatly facilitate the comprehensive knowledge of biological systems. Although imaging methodology for biomacromolecules such as protein and nucleic acids has been long established, microscopic techniques and contrast mechanisms are relatively limited for small biomolecules, which are equally important participants in biological processes. Recent developments in Raman imaging, including both microscopy and tailored vibrational tags, have created exciting opportunities for noninvasive imaging of small biomolecules in living cells, tissues, and organisms. Here, we summarize the principle and workflow of small-biomolecule imaging by Raman microscopy. Then, we review recent efforts in imaging, for example, lipids, metabolites, and drugs. The unique advantage of Raman imaging has been manifested in a variety of applications that have provided novel biological insights.

中文翻译：

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小生物分子的拉曼成像。

成像技术极大地促进了对生物系统的全面了解。尽管用于蛋白质和核酸等生物大分子的成像方法已经建立了很长的时间，但是对于小生物分子来说，显微技术和对比机制相对有限，而小生物分子同样是生物过程中的重要参与者。拉曼成像的最新发展，包括显微镜和定制的振动标签，为活细胞，组织和生物体中的小生物分子的非侵入性成像创造了令人兴奋的机会。在这里，我们总结了通过拉曼显微镜进行小生物分子成像的原理和工作流程。然后，我们回顾了最近在成像方面的努力，例如脂质，代谢物和药物。