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Mucosal-associated invariant T cells and oral microbiome in persistent apical periodontitis.
International Journal of Oral Science ( IF 10.8 ) Pub Date : 2019-05-09 , DOI: 10.1038/s41368-019-0049-y Haleh Davanian 1 , Rogier Aäron Gaiser 1 , Mikael Silfverberg 1 , Luisa W Hugerth 2, 3 , Michał J Sobkowiak 4 , Liyan Lu 1 , Katie Healy 1 , Johan K Sandberg 4 , Peggy Näsman 1 , Jörgen Karlsson 5 , Leif Jansson 5 , Lars Engstrand 2, 3 , Margaret Sällberg Chen 1
International Journal of Oral Science ( IF 10.8 ) Pub Date : 2019-05-09 , DOI: 10.1038/s41368-019-0049-y Haleh Davanian 1 , Rogier Aäron Gaiser 1 , Mikael Silfverberg 1 , Luisa W Hugerth 2, 3 , Michał J Sobkowiak 4 , Liyan Lu 1 , Katie Healy 1 , Johan K Sandberg 4 , Peggy Näsman 1 , Jörgen Karlsson 5 , Leif Jansson 5 , Lars Engstrand 2, 3 , Margaret Sällberg Chen 1
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Opportunistic bacteria in apical periodontitis (AP) may pose a risk for systemic dissemination. Mucosal-associated invariant T (MAIT) cells are innate-like T cells with a broad and potent antimicrobial activity important for gut mucosal integrity. It was recently shown that MAIT cells are present in the oral mucosal tissue, but the involvement of MAIT cells in AP is unknown. Here, comparison of surgically resected AP and gingival tissues demonstrated that AP tissues express significantly higher levels of Vα7.2-Jα33, Vα7.2-Jα20, Vα7.2-Jα12, Cα and tumour necrosis factor (TNF), interferon (IFN)-γ and interleukin (IL)-17A transcripts, resembling a MAIT cell signature. Moreover, in AP tissues the MR1-restricted MAIT cells positive for MR1-5-OP-RU tetramer staining appeared to be of similar levels as in peripheral blood but consisted mainly of CD4+ subset. Unlike gingival tissues, the AP microbiome was quantitatively impacted by factors like fistula and high patient age and had a prominent riboflavin-expressing bacterial feature. When merged in an integrated view, the examined immune and microbiome data in the sparse partial least squares discriminant analysis could identify bacterial relative abundances that negatively correlated with Vα7.2-Jα33, Cα, and IL-17A transcript expressions in AP, implying that MAIT cells could play a role in the local defence at the oral tissue barrier. In conclusion, we describe the presence of MAIT cells at the oral site where translocation of oral microbiota could take place. These findings have implications for understanding the immune sensing of polymicrobial-related oral diseases.
中文翻译：
持续性根尖性牙周炎的黏膜相关不变T细胞和口腔微生物组。
根尖性牙周炎（AP）中的机会细菌可能会引起全身传播的风险。粘膜相关不变T（MAIT）细胞是先天样T细胞，具有广泛而有效的抗菌活性，对肠道粘膜的完整性很重要。最近显示，口腔粘膜组织中存在MAIT细胞，但MAIT细胞是否参与AP尚不清楚。在这里，通过手术切除的AP和牙龈组织的比较表明，AP组织表达的Vα7.2-Jα33，Vα7.2-Jα20，Vα7.2-Jα12，Cα和肿瘤坏死因子（TNF），干扰素（IFN）的水平明显更高-γ和白介素（IL）-17A转录本，类似于MAIT细胞标记。而且，在AP组织中，MR1-5-OP-RU四聚体染色呈阳性的MR1限制性MAIT细胞的水平与外周血相似，但主要由CD4 +子集组成。与牙龈组织不同，AP微生物组受到瘘管和患者高龄等因素的定量影响，并具有突出的表达核黄素的细菌特征。当以整体视图合并时，在稀疏的偏最小二乘判别分析中检查的免疫和微生物组数据可以识别与AP中的Vα7.2-Jα33，Cα和IL-17A转录表达呈负相关的细菌相对丰度，这表明MAIT细胞可能在口腔组织屏障的局部防御中发挥作用。总之，我们描述了口腔微生物群易发生的口腔部位MAIT细胞的存在。
更新日期：2019-05-09
中文翻译：
持续性根尖性牙周炎的黏膜相关不变T细胞和口腔微生物组。
根尖性牙周炎（AP）中的机会细菌可能会引起全身传播的风险。粘膜相关不变T（MAIT）细胞是先天样T细胞，具有广泛而有效的抗菌活性，对肠道粘膜的完整性很重要。最近显示，口腔粘膜组织中存在MAIT细胞，但MAIT细胞是否参与AP尚不清楚。在这里，通过手术切除的AP和牙龈组织的比较表明，AP组织表达的Vα7.2-Jα33，Vα7.2-Jα20，Vα7.2-Jα12，Cα和肿瘤坏死因子（TNF），干扰素（IFN）的水平明显更高-γ和白介素（IL）-17A转录本，类似于MAIT细胞标记。而且，在AP组织中，MR1-5-OP-RU四聚体染色呈阳性的MR1限制性MAIT细胞的水平与外周血相似，但主要由CD4 +子集组成。与牙龈组织不同，AP微生物组受到瘘管和患者高龄等因素的定量影响，并具有突出的表达核黄素的细菌特征。当以整体视图合并时，在稀疏的偏最小二乘判别分析中检查的免疫和微生物组数据可以识别与AP中的Vα7.2-Jα33，Cα和IL-17A转录表达呈负相关的细菌相对丰度，这表明MAIT细胞可能在口腔组织屏障的局部防御中发挥作用。总之，我们描述了口腔微生物群易发生的口腔部位MAIT细胞的存在。
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