The present study has enabled to establish an appropriate kinetic and operational condition for hydrogen production using ultrasonicated palm oil mill effluent (POME). The kinetic analysis was performed corresponds to cumulative hydrogen (H₂) production by varying substrate-inoculum ratio (COD/VS) from 0.2 to 0.6. The maximum H₂ production potential (P) of 448 mL H₂ with lag-time (ρ) of 8.2 h and H₂ production rate (R_m) of 14.62 mL H₂ h⁻¹ was achieved using the substrate-inoculum ratio of 0.6. However, the R_m of 17.979 mL H₂ h⁻¹ with the ρ of 16.84 h, which is almost double than the substrate-inoculum ratio of 0.6 was observed at a substrate-inoculum ratio of 0.4. Furthermore, response surface methodology (RSM), including experimental design, regression analysis, was successfully applied to achieved optimum substrate-inoculum ratio and initial pH for biological H₂ production from ultrasonicated POME. The maximum yield of 0.416 L H₂/g-COD_removal was observed at the optimum conditions of substrate-inoculum ratio of 0.5 and an initial pH of 5.0. The linear, quadratic and interactive effect of substrate-inoculum ratio and initial pH on H₂ yield were significant.

本研究已经能够为使用超声波棕榈油磨机出水（POME）的制氢建立合适的动力学和操作条件。进行动力学分析对应于通过将底物-菌体比（COD / VS）从0.2改变为0.6来累积氢气（H ₂）产生。使用底物与底物的比率为（^-1），获得了448 mL H ₂的最大H ₂产生势（P），滞后时间（ρ）为8.2 h，H ₂产生率（R _m）为14.62 mL H ₂ h ^-1。 0.6。但是，R _m为17.979 mL H ₂ h ^-1ρ为16.84 h，几乎是底物与菌种比0.6的两倍，在底物与菌种比为0.4的情况下。此外，成功地应用了响应表面方法（RSM），包括实验设计，回归分析，以实现最佳的底物-菌体比和初始pH值，以利用超声POME生产生物H ₂。在最佳底物-菌体比例为0.5，初始pH为5.0的最佳条件下，观察到最大_去除量为0.416 LH ₂ / g-COD 。底物-菌种比例和初始pH对H ₂产量的线性，二次和交互作用是显着的。