Gibberellin repression of axillary bud formation in Arabidopsis by modulation of DELLA‐SPL9 complex activity,Journal of Integrative Plant Biology

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Gibberellin repression of axillary bud formation in Arabidopsis by modulation of DELLA‐SPL9 complex activity
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-05-27 , DOI: 10.1111/jipb.12818
Qi‐Qi Zhang ₁ , Jia‐Gang Wang ₁ , Ling‐Yan Wang ₁ , Jun‐Fang Wang ₁ , Qun Wang ₁ , Ping Yu ₁ , Ming‐Yi Bai ₁ , Min Fan ₁

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The formation of lateral branches has an important and fundamental contribution to the remarkable developmental plasticity of plants, which allows plants to alter their architecture to adapt to the challenging environment conditions. The Gibberellin (GA) phytohormones have been known to regulate the outgrowth of axillary meristems (AMs), but the specific molecular mechanisms remain unclear. Here we show that DELLA proteins regulate axillary bud formation by interacting and regulating the DNA‐binding ability of SQUAMOSA‐PROMOTER BINDING PROTEIN LIKE 9 (SPL9), a microRNA156‐targeted squamosa promoter binding protein‐like transcription factor. SPL9 participates in the initial regulation of axillary buds by repressing the expression of LATERAL SUPPRESSOR (LAS), a key regulator in the initiation of AMs, and LAS contributes to the specific expression pattern of the GA deactivation enzyme GA2ox4, which is specifically expressed in the axils of leaves to form a low‐GA cell niche in this anatomical region. Nevertheless, increasing GA levels in leaf axils by ectopically expressing the GA‐biosynthesis enzyme GA20ox2 significantly impaired axillary meristem initiation. Our study demonstrates that DELLA‐SPL9‐LAS‐GA2ox4 defines a core feedback regulatory module that spatially pattern GA content in the leaf axil and precisely control the axillary bud formation in different spatial and temporal.

中文翻译：

赤霉素通过调节DELLA-SPL9复合物活性抑制拟南芥腋芽的形成

侧枝的形成对植物显着的发育可塑性具有重要的根本性贡献，这使植物能够改变其结构以适应具有挑战性的环境条件。已知赤霉素（GA）植物激素可调节腋生分生组织（AM）的生长，但具体的分子机制仍不清楚。在这里我们显示DELLA蛋白通过相互作用和调节SQUAMOSA-PROMOTER BINDING PROTEIN LIKE 9（SPL9）（一种靶向microRNA156的Squamosa启动子结合蛋白样转录因子）的DNA结合能力来调节腋芽的形成。SPL9通过抑制LATERAL SUPPRESSOR（LAS）的表达参与腋芽的初始调控。）是AMs起始的关键调节因子，而LAS则有助于GA失活酶GA2ox4的特定表达模式，该酶在叶腋中特异性表达，从而在此解剖区域中形成低GA细胞位。然而，通过异位表达GA-生物合成酶GA20ox2来增加叶腋中的GA水平会严重损害腋生分生组织的形成。我们的研究表明，DELLA-SPL9-LAS-GA2ox4定义了一个核心反馈调节模块，该模块可在空间上模拟叶腋中GA含量并在不同的时空条件下精确控制腋芽的形成。

更新日期：2019-05-27

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