Waste generation, waste management, sustainable energy production, and global warming are interrelated environmental issues to be considered together. Wastewater treatment sludge is an organic substance rich waste which causes significant environmental problems. However, these wastes can be used as raw material in biofuel generation. This study was designed to investigate the possible utilization of waste sludge in biohydrogen production by taking these facts into consideration. For this purpose, the sludge was first pre-treated with acid and then, the solid (sludge) and liquid (filtrate) phases of acid pre-treated sludge were used as the substrates for biohydrogen generation dark fermentation. Two-factor factorial experimental design method was used in acid hydrolysis of sludge to determine the effect of pH (pH = 2–6) and reaction period (time, min) elution of chemical oxygen demand (COD), total organic carbon (TOC) and total sugar (TS), NH₄N and PO₄P. Statistical evaluation of the results indicated that pH significantly affects the elution of organic carbon and nutrient content of sludge while the reaction time is significant for only organic carbon content. The optimum pretreatment conditions for maximum organic and nutrient elution were determined as pH = 2 and t = 1440 min. The pretreated products, named as filtrate sludge and sludge, conducted to dark fermentation under mesophilic conditions for biohydrogen generation showed that pretreatment of waste sludge at pH = 6 is the best condition giving the maximum yields (Y_H2) as Y_H2 = 24 mmol g⁻¹ Total Sugar _consumed and Y_H2 = 41 mmol g⁻¹ Total sugar consumed, for filtrate and sludge, respectively.

废物产生，废物管理，可持续能源生产和全球变暖是相互关联的环境问题，需要一起考虑。废水处理污泥是富含有机物的废物，会引起严重的环境问题。但是，这些废物可以用作生物燃料生产的原料。这项研究旨在通过考虑这些事实来研究在生物氢生产中废渣的可能利用。为此，首先用酸对污泥进行预处理，然后将酸预处理污泥的固相（污泥）和液相（滤液）用作生物氢生成暗发酵的底物。污泥酸水解采用两因素析因实验设计方法确定pH（pH = 2–6）和反应时间（时间，₄ N和PO ₄的结果的统计P.评价表明，pH值显著影响有机碳和污泥的养分含量的洗脱而反应时间仅用于有机碳含量显著。确定最大有机物和营养物洗脱的最佳预处理条件为pH = 2和t = 1440 min。经过预处理的产物（称为滤液污泥和污泥）在中温条件下进行暗发酵以产生生物氢，表明在pH = 6的条件下对废污泥进行预处理是最佳的条件，因为Y _H2  = 24 mmol g ，其产量最高（Y _H2）^-1总糖_消耗量和Y _H2  = 41 mmol g ^-1 消耗的总糖分，分别用于滤液和污泥。