Intratunical injection of autologous adipose stromal vascular fraction reduces collagen III expression in a rat model of chronic penile fibrosis.,International Journal of Impotence Research

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Intratunical injection of autologous adipose stromal vascular fraction reduces collagen III expression in a rat model of chronic penile fibrosis.
International Journal of Impotence Research ( IF 2.8 ) Pub Date : 2019-04-15 , DOI: 10.1038/s41443-019-0136-9
Lukman Hakim _{1,

2} , Salvatore Fiorenzo ₃ , Petter Hedlund _{4,

5} , Francesco Montorsi ₆ , Trinity J Bivalacqua ₇ , Dirk De Ridder ₁ , Emmanuel Weyne ₁ , David Ralph ₈ , Giulio Garaffa ₈ , Asif Muneer _{8,

9} , Steven Joniau ₁ , Maarten Albersen ₁ , Fabio Castiglione _{1,

6,

8} ,

Affiliation

Previous studies have shown that the injection of adipose stem cells and stromal vascular fraction(SVF) into the tunica albuginea (TA) during the inflammatory phase in a rat model of Peyronie's disease(PD) prevented the development of TA fibrosis. Our aim was to investigate whether local injection of SVF can reduce established fibrosis in a rat model of chronic phase of PD. Eighteen-male 12-wk-old Sprague-Dawley rats were divided in three equal groups: sham, PD without treatment (PD) and PD treated with SVF(PD-SVF). Sham rats underwent 2 injections of vehicle into the TA one month apart. PD rats underwent TGF-β1 injection and injection of vehicle one month later. PD-SVF rats underwent TGF-β1 injection followed by SVF (1-million cells) one month later. One month after the last treatment, the animals, n = 6 rats per group, underwent measurement of intracorporal and mean arterial pressure during electrostimulation of the cavernous nerve. Following euthanasia, penises were harvested for in-vitro study. Erectile function was not statistically significantly different between groups. PD animals developed subtunical areas of fibrosis and elastosis with upregulation of collagen III protein. These fibrotic changes were reversed after injection of SVF. We provide evidence that local injection of SVF reverses TA fibrosis in a rat model of chronic phase of PD.

中文翻译：

在慢性阴茎纤维化的大鼠模型中，自体脂肪间质血管部分的腔内注射降低了胶原III的表达。

先前的研究表明，在Peyronie病（PD）大鼠模型的炎症期，将脂肪干细胞和基质血管部分（SVF）注入白膜膜（TA）可以防止TA纤维化的发展。我们的目的是调查局部注射SVF是否可以减轻PD慢性期大鼠模型中已建立的纤维化。将18只12周大的雄性Sprague-Dawley大鼠分为三个相等的组：假手术，未经治疗的PD（PD）和经SVF治疗的PD（PD-SVF）。假鼠每个月间隔两次向TA注射媒介物。PD大鼠在一个月后进行TGF-β1注射和媒介物注射。PD-SVF大鼠在一个月后进行了TGF-β1注射，然后进行了SVF（100万个细胞）注射。最后一次治疗后一个月，每组n = 6只动物，在海绵状神经电刺激过程中进行体内和平均动脉压的测量。安乐死后，收获阴茎用于体外研究。两组之间的勃起功能在统计学上无显着差异。PD动物出现胶原纤维蛋白上调的纤维膜下和弹性肌膜下区域。注射SVF后，这些纤维化改变被逆转。我们提供的证据表明，在PD慢性期大鼠模型中，局部注射SVF可以逆转TA纤维化。PD动物出现胶原纤维蛋白上调的纤维膜下和弹性肌膜下区域。注射SVF后，这些纤维化改变被逆转。我们提供的证据表明，在PD慢性期大鼠模型中，局部注射SVF可以逆转TA纤维化。PD动物出现胶原纤维蛋白上调的纤维膜下和弹性肌膜下区域。注射SVF后，这些纤维化改变被逆转。我们提供的证据表明，在PD慢性期大鼠模型中，局部注射SVF可以逆转TA纤维化。

更新日期：2019-05-16

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