Cryptochrome-mediated hypocotyl phototropism was regulated antagonistically by gibberellic acid and sucrose in Arabidopsis.,Journal of Integrative Plant Biology

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Cryptochrome-mediated hypocotyl phototropism was regulated antagonistically by gibberellic acid and sucrose in Arabidopsis.
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-05-24 , DOI: 10.1111/jipb.12813
Qing-Ping Zhao ₁ , Jin-Dong Zhu ₁ , Nan-Nan Li ₁ , Xiao-Nan Wang ₁ , Xiang Zhao ₁ , Xiao Zhang ₁

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Both phototropins (phot1 and phot2) and cryptochromes (cry1 and cry2) were proven as the Arabidopsis thaliana blue light receptors. Phototropins predominately function in photomovement, and cryptochromes play a role in photomorphogenesis. Although cryptochromes have been proposed to serve as positive modulators of phototropic responses, the underlying mechanism remains unknown. Here, we report that depleting sucrose from the medium or adding gibberellic acids (GAs) can partially restore the defects in phototropic curvature of the phot1 phot2 double mutants under high‐intensity blue light; this restoration does not occur in phot1 phot2 cry1 cry2 quadruple mutants and nph3 (nonphototropic hypocotyl 3) mutants which were impaired phototropic response in sucrose‐containing medium. These results indicate that GAs and sucrose antagonistically regulate hypocotyl phototropism in a cryptochromes dependent manner, but it showed a crosstalk with phototropin signaling on NPH3. Furthermore, cryptochromes activation by blue light inhibit GAs synthesis, thus stabilizing DELLAs to block hypocotyl growth, which result in the higher GAs content in the shade side than the lit side of hypocotyl to support the asymmetric growth of hypocotyl. Through modulation of the abundance of DELLAs by sucrose depletion or added GAs, it revealed that cryptochromes have a function in mediating phototropic curvature.

中文翻译：

在拟南芥中，隐花色素介导的下胚轴向光性受到赤霉酸和蔗糖的拮抗调节。

向光素（phot1 和 phot2）和隐花色素（cry1 和cry2）均被证明是拟南芥蓝光受体。向光素主要在光运动中发挥作用，隐花色素在光形态发生中发挥作用。尽管隐花色素已被提议作为向光性反应的正调节剂，但其潜在机制仍然未知。在这里，我们报告说，从培养基中耗尽蔗糖或添加赤霉酸（GA）可以部分恢复phot1 phot2双突变体在高强度蓝光下的向光曲率缺陷；这种恢复不会发生在phot1、phot2、cry1、cry2四重突变体和nph3 （ n在向光下胚轴3 上）突变体中，这些突变体在含蔗糖的培养基中向光反应受损。这些结果表明，GA 和蔗糖以隐花色素依赖性方式拮抗地调节下胚轴向光性，但它与 NPH3 上的向光性信号传导存在串扰。此外，蓝光激活的隐花色素抑制GAs合成，从而稳定DELLA以阻止下胚轴生长，导致下胚轴的阴面比亮面的GAs含量更高，从而支持下胚轴的不对称生长。通过消耗蔗糖或添加 GA 来调节 DELLA 的丰度，揭示了隐花色素具有调节向光性曲率的功能。

更新日期：2019-05-24

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