Human embryonic stem cells subjected to a one-time uniaxial stretch for as short as 30-min on a flexible substrate coated with Matrigel experienced rapid and irreversible nuclear-to-cytoplasmic translocation of NANOG and OCT4, but not Sox2. Translocations were directed by intracellular transmission of biophysical signals from cell surface integrins to nuclear CRM1 and were independent of exogenous soluble factors. On E-CADHERIN-coated substrates, presumably with minimal integrin engagement, mechanical strain-induced rapid nuclear-to-cytoplasmic translocation of the three transcription factors. These findings might provide fundamental insights into early developmental processes and may facilitate mechanotransduction-mediated bioengineering approaches to influencing stem cell fate determination.

中文翻译：

---

多能性相关转录因子通过单轴外力快速移位。

人类胚胎干细胞在涂有Matrigel的柔性基底上进行了一次单轴拉伸，短至30分钟，经历了NANOG和OCT4的快速且不可逆的核到细胞质移位，但没有Sox2。易位是由生物物理信号从细胞表面整联蛋白到核CRM1的胞内传递而引起的，并且与外源可溶性因子无关。在E-CADHERIN涂层的底物上，可能具有最小的整联蛋白参与，机械应变诱导了三种转录因子的快速核质转移。这些发现可能提供对早期发育过程的基础见解，并可能促进机械转导介导的生物工程方法影响干细胞命运的确定。