Pre-mRNA 3'-end maturation is achieved by a mechanism requiring four different protein complexes assembled from approximately twenty factors. A global understanding of this essential process is still missing due to the inability to structurally characterize the entire complexes, even though structures of the isolated factors have been obtained. In this review, we summarize recent findings regarding the atomic description of one of the major players, the Cleavage and Polyadenylation Specificity Factor complex (CPSF in human, CPF in yeast). These data provide information on the architecture adopted by the major components of this complex, and on its capacity to recognize the polyadenylation signal sequence.

中文翻译：

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3'端mRNA成熟机制的结构见解：聚腺苷酸化信号识别的快照。

mRNA前3'端的成熟是通过一种机制来实现的，该机制需要从大约二十个因子中组装出四种不同的蛋白质复合物。由于无法从结构上表征整个复合物，因此即使获得了分离因子的结构，也仍然缺乏对该基本过程的全局理解。在这篇综述中，我们总结了有关主要参与者之一的原子描述的最新发现，即裂解和聚腺苷酸化特异性因子复合物（人中的CPSF，酵母中的CPF）。这些数据提供了有关该复合物主要成分所采用的体系结构及其识别聚腺苷酸化信号序列的能力的信息。