OBJECTIVES The potential implication of micro-RNAs (miRs) in the negative association between diabetes and abdominal aortic aneurysm (AAA) has so far never been addressed. The aim of this study was to compare miR expression between diabetic and non-diabetic patients with AAA. METHODS Ten diabetic patients were prospectively included and compared to 10 age- and sex-matched non-diabetic patients with infrarenal AAA. A profiling analysis of 752 human miRs was performed from peripheral blood mononuclear cells (PBMCs) using miRCURY LNA Universal RT microRNA PCR (Exiqon- Qiagen®). miR that showed significant differential expression (P < 0.05) were selected and further analyzed in the entire cohort in sera, plasma and aneurysmal aortic tissues. RESULTS Four miRs were significantly differentially expressed in PBMCs of diabetic patients compared to non-diabetics: 3 were upregulated (miR-144-3p, 20a-5p and 188-3p) and 1 downregulated (miR-548k). miR-144-3p and miR-548k were also increased in aneurysmal tissue and miR-20a-5p was increased in serum. The expression of miR-20a-5p in PBMCs was correlated with fructosamine concentration (r = 0.62, p = 0.006). CONCLUSIONS Even if further studies are required to determine their direct role in AAA, these miRs could represent interesting new targets.

中文翻译：

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糖尿病患者腹主动脉瘤的微RNA差异表达。

目的迄今为止，尚未解决micro-RNA（miRs）在糖尿病与腹主动脉瘤（AAA）之间负相关的潜在意义。这项研究的目的是比较糖尿病和非糖尿病AAA患者的miR表达。方法前瞻性纳入10例糖尿病患者，并将其与10例年龄和性别相匹配的非糖尿病肾下AAA患者进行比较。使用miRCURY LNA通用RT microRNA PCR（Exiqon-Qiagen®）从外周血单核细胞（PBMC）进行了752条人类miR的分析分析。选择显示显着差异表达（P <0.05）的miR，并在血清，血浆和动脉瘤主动脉组织的整个队列中进行进一步分析。结果与非糖尿病患者相比，糖尿病患者的PBMC中有四个miRs显着差异表达：3个上调（miR-144-3p，20a-5p和188-3p）和1个下调（miR-548k）。动脉瘤组织中的miR-144-3p和miR-548k也升高，血清中的miR-20a-5p也升高。PBMC中miR-20a-5p的表达与果糖胺浓度相关（r = 0.62，p = 0.006）。结论即使需要进一步研究确定其在AAA中的直接作用，这些miR仍可能代表有趣的新靶标。006）。结论即使需要进一步研究确定其在AAA中的直接作用，这些miR仍可能代表有趣的新靶标。006）。结论即使需要进一步研究确定其在AAA中的直接作用，这些miR仍可能代表有趣的新靶标。