The fungal species Trichoderma is reported to have a significant impact on the growth and physiological performance of rice plants. However, the molecular mechanisms that induce these effects remain unspecified. Using next-generation sequencing technology, this study compared the differential expression of genes in rice seedlings that had been inoculated with Trichoderma asperellum SL2 with the gene expression in seedlings that had no such inoculation. The study showed that many genes related to plant growth enhancement and physiological functioning are differentially expressed in seedlings which have been symbiotically colonized by T. asperellum SL2. In these seedlings, specific genes related to photosynthesis, RNA activity, stomatal activity, and root development were found to be up-regulated as others were down-regulated. Although the exact causal mechanisms at the molecular level remain to be identified, the presence of Trichoderma versus its absence was associated with almost ten times more significant up-regulations than down-regulations for specific genes that have been identified from previous genomic mapping. Such analysis at the molecular level can help to explain observed phenotypic effects at the organismic level, and it begins to illuminate the observed beneficial relationships expressed phenotypically between crop plants and certain symbiotic microbes.

中文翻译：

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共生真菌 Trichoderma asperellum SL2 接种水稻幼苗的转录组学分析

据报道，真菌物种木霉对水稻植物的生长和生理性能有显着影响。然而，诱导这些影响的分子机制仍未明确。本研究利用二代测序技术，比较了接种木霉SL2的水稻幼苗中基因的差异表达与未接种这种菌的水稻幼苗中的基因表达差异。该研究表明，许多与植物生长促进和生理功能相关的基因在已被 T. asperellum SL2 共生定殖的幼苗中差异表达。在这些幼苗中，发现与光合作用、RNA 活性、气孔活性和根发育相关的特定基因上调，而其他基因下调。虽然分子水平上的确切因果机制仍有待确定，但木霉的存在与不存在相比，与先前基因组图谱中确定的特定基因的显着上调相比，其显着上调几乎是下调的 10 倍。这种分子水平的分析有助于解释在生物体水平上观察到的表型效应，它开始阐明观察到的作物植物与某些共生微生物之间表型表达的有益关系。