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Magnetic particles as Powerful Purification Tool for High Sensitive Mass Spectrometric Screening Procedures
Proteomics ( IF 3.4 ) Pub Date : 2010-08-19 , DOI: 10.1002/pmic.200800535
Jochen F. Peter 1 , Angela M. Otto 2 , Bernhard Wolf 2
Affiliation  

The effective isolation and purification of proteins from biological fluids is the most crucial step for a successful protein analysis when only minute amounts are available. While conventional purification methods like dialysis, ultrafiltration or protein precipitation often leads to a marked loss of protein, solid phase extraction (SPE) with small‐sized particles is a powerful alternative. The implementation of particles with superparamagnetic cores facilitates the handling of those particles and allows the application of particles in the nm‐ to low ìm range.

中文翻译:

磁性粒子作为高灵敏度质谱筛查程序的有力纯化工具

如果只有少量样品,从生物流体中有效分离和纯化蛋白是成功进行蛋白分析的最关键步骤。尽管常规的纯化方法(例如透析,超滤或蛋白质沉淀)通常会导致蛋白质明显损失,但使用小颗粒的固相萃取(SPE)是一种有力的选择。具有超顺磁性核的颗粒的实施有利于这些颗粒的处理,并允许在nm至低微米范围内应用颗粒。
更新日期:2019-11-18
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