De novo root regeneration (DNRR) has wide applications in agriculture such as those related to cutting technology. Detached Arabidopsis thaliana leaf explants can regenerate adventitious roots without added hormones. The regenerative ability is highly dependent on the developmental status of the leaf. An immature leaf has a higher regenerative ability, while a mature leaf is difficult to regenerate. Using RNA-Seq analysis, we showed that the expression levels of many genes, including those in the auxin network, changed during leaf maturation. Particularly, the expression levels of many YUCCA (YUC) genes in the auxin biosynthesis pathway are responsive to leaf maturation. Overexpression of YUC1 in the yuc-1D dominant mutant rescued the rooting defects caused by leaf maturation. In addition, YUC4 expression levels were also affected by circadian rhythms. The regenerative ability was reduced in both immature and mature mutant leaf explants from the new wuschel-related homeobox 11-3 (wox11-3) and wox12-3 mutant alleles created by the CRISPR/Cas9 method. Overall, the transcriptome and genetic data, together with the auxin concentration analysis, indicate that the ability to upregulate auxin levels upon detachment may be reduced during leaf maturation. Thus, multiple developmental and environmental signals may converge to control auxin accumulation, which affects the efficiency of the WOX11/12-mediated DNRR from leaf explants.

从头再生（DNRR）在农业中具有广泛的应用，例如与切割技术相关的应用。分离的拟南芥叶外植体可以再生不定根，而无需添加激素。再生能力高度依赖于叶片的发育状态。不成熟的叶片具有较高的再生能力，而成熟的叶片则难以再生。使用RNA-Seq分析，我们显示了许多基因的表达水平，包括生长素网络中的那些基因，在叶片成熟过程中发生了变化。特别是，许多表达水平丝兰（YUC在生长素生物合成途径）的基因响应于叶成熟。过表达YUC1在YUC-1D显性突变体挽救了由叶片成熟引起的生根缺陷。另外，YUC4表达水平也受昼夜节律影响。在通过CRISPR / Cas9方法创建的新的乌谢尔相关的同源盒11-3（wox11-3）和wox12-3突变等位基因中，未成熟和成熟的突变叶片外植体的再生能力均降低。总体而言，转录组和遗传数据以及植物生长素浓度分析表明，叶片成熟过程中分离时上调植物生长素水平的能力可能会降低。因此，多种发育和环境信号可能会收敛以控制植物生长素的积累，从而影响WOX11 / 12的效率。外植体介导的DNRR。