RATIONALE Assessing the utility of vacuum matrix-assisted ionization (MAI) for the direct and rapid analysis of lipids in complex samples with emphasis on bacterial taxonomy. METHODS Matrix-assisted ionization Fourier transform mass spectrometry (MAI-FTMS) was used to characterize polar and non-polar lipids in mixtures. RESULTS For non-polar lipid triacylglycerols (TAGs), MAI-FTMS produced lipid-specific ions for eight different edible oils and allowed these oils to be identified based on their MAI-FTMS profiles. For polar lipids from bacteria, MAI-FTMS of crude lipid extracts allowed taxonomic identification of eight blind-coded samples based on taxonomy-specific phospholipid profiles. MAI produced results comparable and complementary to benchmark MALDI and ESI methods currently used for characterization of polar and non-polar lipids in the same mixtures. CONCLUSIONS The newly developed MAI technique is a rapid, simple and complementary method for the characterization of polar and non-polar lipids in complex mixtures.

中文翻译：

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用于分析脂质的基质辅助电离傅立叶变换质谱法。

原理 评估真空基质辅助电离 (MAI) 在复杂样品中直接和快速分析脂质的效用，重点是细菌分类。方法 基质辅助电离傅里叶变换质谱 (MAI-FTMS) 用于表征混合物中的极性和非极性脂质。结果 对于非极性脂质三酰甘油 (TAGs)，MAI-FTMS 为八种不同的食用油产生了脂质特异性离子，并允许根据其 MAI-FTMS 谱对这些油进行鉴定。对于来自细菌的极性脂质，粗脂质提取物的 MAI-FTMS 允许基于分类特定的磷脂谱对八种盲编码样品进行分类鉴定。MAI 产生的结果与目前用于表征相同混合物中极性和非极性脂质的基准 MALDI 和 ESI 方法相当并互补。结论 新开发的 MAI 技术是一种快速、简单和互补的方法，用于表征复杂混合物中的极性和非极性脂质。