BACKGROUND Catheter-associated urinary tract infections (CAUTIs) are among the most common hospital-acquired infections, leading to increased morbidity and mortality. A major reason for this is that urinary catheters are not yet capable of preventing CAUTIs. AIM To develop an anti-infective urinary catheter. METHODS An efficient silver-polytetrafluoroethylene (Ag-PTFE) nanocomposite coating was deposited on whole silicone catheters, and two in-vitro bladder models were designed to test antibacterial (against Escherichia coli) and anti-encrustation (against Proteus mirabilis) performances. Each model was challenged with two different concentrations of bacterial suspension. FINDINGS Compared with uncoated catheters, coated catheters significantly inhibited bacterial migration and biofilm formation on the external catheter surfaces. The time to develop bacteriuria was an average of 1.8 days vs 4 days and 6 days vs 41 days when the urethral meatus was infected with 106 and 102 cells/mL, respectively. For anti-encrustation tests, the coated catheter significantly resisted encrustation, although it did not strongly inhibit the increases in bacterial density and urinary pH. The time to blockage, which was found to be independent of the initial bacterial concentration in the bladder, was extended from 36.2±1.1 h (uncoated) to 89.5±3.54 h (coated) following bacterial contamination with 103 cells/mL in the bladder. Moreover, the coated catheter exhibited excellent biocompatibility with L929 fibroblast cells. CONCLUSION Ag-PTFE coated Foley catheters should undergo further clinical trials to determine their ability to prevent CAUTIs during catheterization.

中文翻译：

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银-聚四氟乙烯纳米复合涂层导尿管的体外抗菌和防结壳性能。

背景技术与导管相关的尿路感染（CAUTI）是最常见的医院获得性感染，导致发病率和死亡率增加。主要原因是导尿管尚不能预防CAUTI。目的开发抗感染导尿管。方法将有效的银-聚四氟乙烯（Ag-PTFE）纳米复合涂层沉积在整个硅胶导管上，并设计了两种体外膀胱模型来测试抗菌性能​​（对抗大肠埃希氏菌）和抗结壳性能（对抗变形杆菌）。用两种不同浓度的细菌悬浮液攻击每个模型。结果与未涂覆的导管相比，涂覆的导管显着抑制了细菌迁移和导管外表面上的生物膜形成。当尿道口分别感染106和102细胞/ mL时，细菌尿的形成时间平均为1.8天，4天和6天与41天。对于抗结壳测试，带涂层的导管虽然不能强烈抑制细菌密度和尿液pH值的增加，但可以显着抵抗结壳。发现阻塞时间与膀胱中的初始细菌浓度无关，在细菌被膀胱中的103个细胞/ mL污染后，阻塞时间从36.2±1.1 h（未包衣）延长至89.5±3.54 h（包衣）。此外，带涂层的导管与L929成纤维细胞表现出极好的生物相容性。结论Ag-PTFE涂层的Foley导管应进行进一步的临床试验，以确定其在导管插入期间预防CAUTI的能力。