miR164c and miR168a regulate seed vigor in rice,Journal of Integrative Plant Biology

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miR164c and miR168a regulate seed vigor in rice
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2019-04-04 , DOI: 10.1111/jipb.12792
Yan Zhou ₁ , Shiqi Zhou ₁ , Liping Wang _{1,

2} , Duo Wu ₁ , Hailan Cheng ₁ , Xu Du ₁ , Dandan Mao ₁ , Chunlai Zhang ₃ , Xiaocheng Jiang ₁

Affiliation

MicroRNAs (miRNAs) are key regulators of gene expression in many important biological processes of plants. However, few miRNAs have been shown to regulate seed vigor. Here, we conducted microarray assays to analyze miRNA expression levels in seeds of the rice (Oryza sativa L.) cultivar ZR02. Results showed significant differences in the expression of 11 miRNAs between artificially aged and untreated control seeds. Among these, osa‐miR164c was transcriptionally upregulated, while osa‐miR168a was downregulated in artificially aged seeds; this was verified by quantitative real‐time PCR analysis. Under the same aging condition, osa‐miR164c overexpression in OE164c transgenic seeds and osa‐miR168a silencing in MIM168a transgenic seeds of the rice cultivar Kasalath led to lower germination rates, whereas osa‐miR164c silencing in MIM164c and osa‐miR168a overexpression in OE168a resulted in higher seed germination rates compared with wild‐type seeds. Meanwhile, changes in cytomembrane permeability of seeds and in the expression level of osa‐miR164c target genes (OsPM27 and OsPSK5) and osa‐miR168a target genes (OsAGO1 and OsPTR2) under aging conditions coincided with changes in seed vigor induced by osa‐miR164c and osa‐miR168a. Thus, genetic manipulation of miRNAs has important implications in the development of crop cultivars with high vigor and extended life span of seeds.

中文翻译：

miR164c和miR168a调节水稻的种子活力

微小RNA（miRNA）是植物许多重要生物学过程中基因表达的关键调节因子。但是，很少有miRNA能够调节种子活力。在这里，我们进行了微阵列分析，以分析水稻（Oryza sativa L.）品种ZR02种子中的miRNA表达水平。结果显示，人工老化和未处理的对照种子之间11种miRNA的表达存在显着差异。在这些中，OSA-miR164c被转录上调，而OSA-miR168a在人工老化种子下调; 这已通过实时定量PCR分析进行了验证。在相同的衰老条件下，osa-miR164c在OE164c转基因种子和osa-miR168a中过表达水稻品种Kasalath的MIM168a转基因种子中的沉默导致较低的发芽率，而MIM164c中的osa-miR164c沉默和OE168a中的osa-miR168a过表达导致种子发芽率高于野生型种子。同时，改变种子的细胞膜渗透性和在表达水平OSA-miR164c靶基因（OsPM27和OsPSK5）和OSA-miR168a靶基因（OsAGO1和OsPTR2老化条件下）与由诱导种子活力的变化一致OSA-miR164c和osa‐miR168a。因此，miRNA的基因操作对具有高活力和延长种子寿命的农作物品种的发展具有重要意义。

更新日期：2019-04-04

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