Organic micropollutants like pharmaceuticals, personal care products, pesticides and industrial chemicals have received an ever growing attention due to their adverse ecological effects in natural water bodies. A promising technology to remove the micropollutants from the wastewater is biocatalytic treatment using oxidoreductase enzymes. In order to improve removal efficiency and feasibility, the focus has been on developing enzyme-mediator systems and immobilisation techniques. Nonetheless, since oxidoreductase enzymes often promote coupling reactions, enzymatic reactions do not necessarily result in simpler compounds. Thus transformation products, which might be in equally or even more hazardous pollutants, can be still present in the wastewater after treatment. Therefore, the toxicity and ecological effects of the reaction products have to be investigated rigorously to ensure that the treatment mitigates the environmental and health related impacts.

The objective of this paper is to give an overview of research carried out with oxidoreductase enzymes to treat different organic micropollutants and their mixtures along with real wastewater and to collect available information on different techniques, transformation products and toxicity. Though in most cases the reviewed enzymatic treatment methods resulted in less toxic effluent, the application of mediators, while facilitating higher transformation rates, increased the level of toxicity. Hence, it was concluded that the suitability of biocatalytic methods should be assessed from the perspective of toxicity beside the removal efficiency in order to allow the upscaling of the technology to a feasible and sustainable wastewater treatment solution.

诸如药物，个人护理产品，农药和工业化学品之类的有机微污染物由于其对天然水体的不利生态影响而受到越来越多的关注。一种从废水中去除微量污染物的有前途的技术是使用氧化还原酶的生物催化处理。为了提高去除效率和可行性，重点一直放在开发酶介体系统和固定化技术上。但是，由于氧化还原酶通常促进偶联反应，因此酶促反应不一定会产生更简单的化合物。因此，处理后的废水中仍可能存在转化产物，这些转化产物可能含有同等甚至更多的有害污染物。所以，

本文的目的是概述使用氧化还原酶处理不同有机微污染物及其混合物以及实际废水的研究，并收集有关不同技术，转化产物和毒性的可用信息。尽管在大多数情况下，经过审查的酶处理方法所产生的废水毒性较小，但在促进较高转化率的同时，使用介体可提高毒性水平。因此，得出的结论是，应从除去除效率之外的毒性的角度评估生物催化方法的适用性，以使该技术升级为可行且可持续的废水处理解决方案。