Long-term accumulation of organophosphate pesticides in environment presents a potential hazard to human and animal health. Towards this, a highly sensitive amperometric AChE-biosensor based on conjugated polymer and Ag-rGO-NH₂ nanocomposite has been successfully developed. First, 4, 7-di (furan-2-yl) benzo thiadiazole (FBThF) was electrochemically polymerized on the electrode surface. Then, Ag-rGO-NH₂ nanocomposite and acetylcholinesterase (AChE) are modified on the polymer membrane surface. In this way, a novel amperometric AChE-biosensor was successfully prepared. The as-prepared biosensor possessed excellent conductivity, catalytic activity, and biocompatibility which were attributed to the synergistic effects of poly(FBThF) and Ag-rGO-NH₂ and provided a hydrophilic surface for AChE adhesion. Under optimized conductions, the linear range was 0.099–9.9 μg L⁻¹ with a regression coefficient of 0.9947 for malathion, 0.0206–2.06 μg L⁻¹ with a regression coefficient of 0.9969 for trichlorfon. The detection limit is calculated to be about 0.032 μg L⁻¹ for malathion and 0.001 μg L⁻¹ for trichlorfon (S/N = 3). Moreover, the biosensor exhibited acceptable reproducibility and long-term stability, which makes it possible to provide a novel and promising tool for analysis of organophosphate pesticides.

有机磷酸酯农药在环境中的长期积累对人类和动物健康构成潜在危害。为此，已经成功地开发了基于共轭聚合物和Ag-rGO-NH ₂纳米复合材料的高灵敏度电流型AChE生物传感器。首先，在电极表面上电化学聚合4，7-二（呋喃-2-基）苯并噻二唑（FBThF）。然后，将Ag-rGO-NH ₂纳米复合材料和乙酰胆碱酯酶（AChE）修饰在聚合物膜表面上。以这种方式，成功地制备了新型的电流型AChE生物传感器。所制备的生物传感器具有优异的电导率，催化活性和生物相容性，这归因于聚（FBThF）和Ag-rGO-NH ₂的协同作用。并为AChE粘合提供了亲水性表面。在最佳传导条件下，马拉硫磷的线性范围为0.099–9.9μgL ^-1，三氯敌草的线性回归系数为0.9947；三氯敌草的线性范围为0.0206–2.06μgL ^-1，回归系数为0.9969。检出限为计算为约0.032微克大号^-1对马拉硫磷和0.001微克大号^-1为敌百虫（S / N = 3）。而且，该生物传感器表现出可接受的再现性和长期稳定性，这使得有可能提供一种用于分析有机磷酸盐农药的新颖且有前途的工具。