E. pisi is an ascomycete member causing powdery mildew disease of garden pea. It is a biotrophic pathogen, requiring a living host for its survival. Our understanding of molecular mechanisms underlying its pathogenesis is limited. The identification of proteins expressed in the pathogen is required to gain an insight into the functional mechanisms of an obligate biotrophic fungal pathogen. In this study, the proteome of the anamorphic stage of E. pisi pathogen has been elucidated through the nano LC–MS/MS approach. A total of 328 distinct proteins were detected from Erysiphe isolates infecting the susceptible pea cultivar, Arkel. The proteome is available via ProteomeXchange with identifier PXD010238. The functional classification of protein accessions based on Gene Ontology revealed proteins related to signal transduction, secondary metabolite formation and stress which might be involved in virulence and pathogenesis. The functional validation carried through differential expression of genes encoding G-protein beta subunit, a Cyclophilin (Peptidyl prolyl cis-transisomerase) and ABC transporter in a time course study confirmed their putative role in pathogenesis between resistant and susceptible genotypes, JI2480 and Arkel. The garden pea-powdery mildew pathosystem is largely unexplored, therefore, the identified proteome provides a first-hand information and will form a basis to analyze mechanisms involving pathogen survival, pathogenesis and virulence.

E. pisi是引起豌豆白粉病的子囊菌成员。它是一种生物营养病原体，需要活宿主才能生存。我们对其发病机制的分子机制的理解是有限的。为了深入了解专性生物营养真菌病原体的功能机制，需要鉴定病原体中表达的蛋白质。在本研究中，通过纳米 LC-MS/MS 方法阐明了E. pisi病原体变形阶段的蛋白质组。从感染易感豌豆品种 Arkel 的白粉菌分离株中检测到总共 328 种不同的蛋白质。蛋白质组可通过 ProteomeXchange 获得，标识符为 PXD010238。基于基因本体论的蛋白质功能分类揭示了与信号转导、次级代谢物形成和应激相关的蛋白质，这些蛋白质可能参与毒力和发病机制。在一项时间过程研究中，通过编码 G 蛋白 β 亚基、亲环蛋白（肽基脯氨酰顺反异构酶）和 ABC 转运蛋白的基因的差异表达进行功能验证，证实了它们在抗性和易感基因型 JI2480 和 Arkel 之间发病机制中的假定作用。豌豆白粉病的病理系统在很大程度上尚未被探索，因此，鉴定的蛋白质组提供了第一手信息，并将成为分析病原体生存、发病机制和毒力机制的基础。