A facile one-step electrochemical synthesis of a platinum/reduced graphene oxide/poly(3-aminobenzoic acid) (Pt/rGO/P3ABA) nanocomposite film on a screen-printed carbon electrode (SPCE) and its application in the development of sensitive amperometric biosensors was successfully demonstrated herein. The electropolymerization of P3ABA together with co-electrodeposition of rGO and Pt was conducted by cyclic voltammetry, as was the GO reduction to rGO. A Pt/rGO/P3ABA-modified SPCE exhibited excellent electrocatalytic oxidation towards hydrogen peroxide (H₂O₂) and can be employed as an electrochemical platform for the immobilization of glucose oxidase (GOx) and cholesterol oxidase (ChOx) to fabricate glucose and cholesterol biosensors, respectively. Under the optimized conditions at a working potential of +0.50 V, the proposed biosensors revealed excellent linear responses to glucose and cholesterol in the concentration ranges of 0.25–6.00 mM and 0.25–4.00 mM, respectively, with high sensitivities of 22.01 and 15.94 μA mM⁻¹ cm⁻² and low detection limits (LODs) of 44.3 and 40.5 μM. Additionally, the Michaelis-Menten constant (K_m) of GOx was 3.54 mM, while the K_m of ChOx was 3.82 mM. Both biosensors displayed a good anti-interference ability and clearly exhibited acceptable recoveries for the detection of glucose and cholesterol in a human serum sample (98.2–104.1%).

丝网印刷碳电极（SPCE）上铂/还原氧化石墨烯/聚（3-氨基苯甲酸）（Pt / rGO / P3ABA）纳米复合膜的简便一步电化学合成及其在灵敏安培计开发中的应用本文成功地证明了生物传感器。P3ABA的电聚合以及rGO和Pt的共电沉积是通过循环伏安法进行的，GO还原为rGO也是如此。Pt / rGO / P3ABA改性的SPCE对过氧化氢（H ₂ O ₂）并可用作固定葡萄糖氧化酶（GOx）和胆固醇氧化酶（ChOx）的电化学平台，以分别制造葡萄糖和胆固醇生物传感器。在优化的条件下，+ 0.50 V的工作电势下，拟议的生物传感器在浓度范围分别为0.25–6.00 mM和0.25–4.00 mM时对葡萄糖和胆固醇表现出出色的线性响应，并具有22.01和15.94μAmM的高灵敏度。^-1  cm ^-2和44.3和40.5μM的低检测限（LOD）。此外，GOx的Michaelis-Menten常数（K _m）为3.54 mM，而K _mChOx的浓度为3.82 mM。两种生物传感器均显示出良好的抗干扰能力，并且在检测人血清样品中的葡萄糖和胆固醇方面具有明显的可接受的回收率（98.2–104.1％）。