In the study, the neuroprotectivities of forsythiaside, a main constituent of Forsythia suspensa (Thunb.) Vahl (F. suspensa, Lianqiao in Chinese), were investigated in the hippocampal slices. Forsythiaside suppressed the overexpression of cyclooxygenase-2 (COX-2) and monoacylglycerol lipase (MAGL) proteins induced by β-amyloid (Aβ25-35) to upregulate the levels of 2-arachidonoylglycerol (2-AG), an endogenous endocannabinoids. Then the inhibition of forsythiaside on COX-2 was deeply studied by the molecular docking. Forsythiaside prevented neuroinflammation and apoptosis from Aβ25-35 insults, and this action appeared to be mediated via cannabinoid receptor 1 (CB1R)-dependent nuclear factor-κB (NF-κB) signaling pathways. More importantly, forsythiaside functionally improved Aβ25-35-induced learning and memory deficits, which was indicated by long term potentiation (LTP). Taken together, forsythiaside may have therapeutic potential for Alzheimer's diseases (AD) by increasing the levels of 2-AG.

中文翻译：

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连翘苷可通过大麻素受体1依赖性NF-κB通路上调2-花生四烯酰甘油来预防β淀粉样蛋白诱导的海马切片损伤。

在这项研究中，在海马切片中研究了连翘的神经保护性，连翘的主要成分是连翘（Thunb。）Vahl（F. suspensa，中文，连桥）。连翘苷抑制了β-淀粉样蛋白（Aβ25-35）诱导的环氧合酶-2（COX-2）和单酰基甘油脂酶（MAGL）蛋白的过表达，从而上调了内源性内源性大麻素2-花生四烯酰基甘油（2-AG）的水平。然后通过分子对接深入研究了连翘苷对COX-2的抑制作用。连翘苷预防了Aβ25-35损伤引起的神经炎症和细胞凋亡，并且该作用似乎是通过大麻素受体1（CB1R）依赖性核因子-κB（NF-κB）信号传导途径介导的。更重要的是，连翘苷在功能上改善了Aβ25-35诱导的学习和记忆障碍，长期增强（LTP）指示了这一点。总体而言，连翘苷可能通过增加2-AG的水平而具有治疗阿尔茨海默氏病（AD）的潜力。