The endophytic fungus Phomopsis liquidambari play a key role in habitat adaptation of rice (Oryza sativa L.) with potential multiple beneficial. However, our previous published work on this subject remains incomplete. Here, we performed a soil nutrient (nitrogen and phosphorus) transformation with related functional genes and elucidated how rhizosphere microbiota vary their response to P. liquidambari interaction throughout the plant’s life cycle under field conditions by Illumina Miseq sequencing platforms in a nutrient-limited paddy soil. Our results showed that P. liquidambari symbiosis decreased the nitrogen and phosphorus loss by 24.59% and 17.46% per pot, respectively. Additionally, we suggest that the application of P. liquidambari altered the activation of soil nitrogen and phosphorus functional genes to accelerate nutrient turnover in the rice rhizosphere. High-throughput sequencing with co-occurrence network and species-related network analysis further revealed that P. liquidambari colonization influenced the patterns of microbiota shift in the rhizosphere, especially during the heading stages. This led to an optimized microbial community through the promotion and inhibition of indigenous soil microbes with a higher level of available nutrient supplies. Our study strongly proposes rice-P. liquidambari symbiosis as a useful candidate for improving N and P acquisition and utilization.

内生真菌液体拟南芥（Phomopsis liquidambari）在水稻（Oryza sativa L.）的生境适应中起着关键作用，具有潜在的多重效益。但是，我们先前在该主题上发表的工作仍不完整。在这里，我们利用Illumina Miseq测序平台在营养有限的稻田中，利用相关功能基因对土壤的养分（氮和磷）进行了转化，并阐明了根际微生物群如何在田间条件下改变其在整个植物生命周期中对P.liquidambari相互作用的响应。。我们的研究结果表明，每盆液liquid共生共生氮和磷的流失量分别降低了24.59％和17.46％。另外，我们建议拟南芥改变了土壤氮和磷功能基因的活化，从而加速了水稻根际的养分周转。通过共现网络和物种相关网络分析进行的高通量测序进一步揭示了液态拟杆菌的定殖影响了根际微生物区系转移的模式，特别是在抽穗期。通过促进和抑制具有较高可用营养物供应水平的本地土壤微生物，从而优化了微生物群落。我们的研究强烈建议将稻-P.liquidambari共生作为改善氮和磷获取与利用的有用候选者。