Abstract Naturally derived Hydroxyapatite (HAp) from fish scale is finding wide applications in the development of bone scaffold to promote bone regeneration. But porous HAp scaffold is fragile in nature making it unsuitable for bone repair or replacement applications. Thus, it is essential to improve the mechanical property of HAp scaffolds while retaining the interconnected porous structure for tissue ingrowth in vivo. In this study solvent casting particulate leaching technique is used to develop novel Puntius conchonius fish scale derived HAp bone scaffold by varying the wt.% of the HAp from 60 to 80% in PMMA matrix. Physico-chemical, mechanical, structural and bioactive properties of the developed scaffolds are investigated. The obtained results indicate that HAp-PMMA scaffold at 70 wt % HAp loading shows optimal properties with 7.26 ± 0.45 MPa compressive strength, 75 ± 0.8% porosity, 8.0 ± 0.68% degradation and 190 ± 11% water absorption. The obtained results of the scaffold can meet the physiological demands to guide bone regeneration. Moreover, in vitro bioactivity analysis also confirms the formation of bone like apatite in the scaffold surface after 28 days of SBF immersion. Thus, the developed scaffold has the potential to be effectively used in bone tissue engineering applications.

中文翻译：

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使用 Puntius conchonius 鱼鳞衍生的羟基磷灰石开发骨支架：物理力学和生物活性评估

摘要 从鱼鳞中天然衍生的羟基磷灰石（HAp）在骨支架的开发中得到了广泛的应用，以促进骨再生。但多孔 HAp 支架本质上是脆弱的，因此不适合骨修复或置换应用。因此，提高 HAp 支架的机械性能，同时保留互连的多孔结构以促进体内组织向内生长至关重要。在这项研究中，溶剂浇铸颗粒浸出技术用于通过改变 PMMA 基质中 HAp 的重量百分比从 60% 到 80% 来开发新型 Puntius conchonius 鱼鳞衍生的 HAp 骨支架。研究了所开发支架的物理化学、机械、结构和生物活性特性。获得的结果表明 HAp-PMMA 支架在 70 wt% HAp 负载下显示出最佳性能，为 7.26 ± 0。45 MPa 抗压强度、75 ± 0.8% 孔隙率、8.0 ± 0.68% 降解和 190 ± 11% 吸水率。该支架获得的结果可以满足指导骨再生的生理需求。此外，体外生物活性分析也证实了在 SBF 浸泡 28 天后支架表面形成了类似骨的磷灰石。因此，开发的支架有可能有效地用于骨组织工程应用。