Analytical microarrays feature great capabilities for simultaneous detection and quantification of multiple analytes in a single measurement. In this work, we present a rapid and simple method for bulk preparation of microarrays on polycarbonate sheets. Succinylated Jeffamine® ED-2003 was screen printed on polycarbonate sheets to create a polyfunctional shielding layer by baking at 100 °C. After microdispension of capture probes (antibodies, oligonucleotides, or small molecules) in a microarray format, chips were assembled with a flow cell from double-sided tape. It was shown that the shielding layer was firmly coated and suppressed unspecific binding of proteins. Universal applicability was demonstrated by transferring established flow-based chemiluminescence microarray measurement principles from glass slides to polycarbonate chips without loss of analytical performance. Higher chemiluminescence signals could be generated by performing heterogeneous asymmetric recombinase polymerase amplification on polycarbonate chips. Similar results could be shown for sandwich microarray immunoassays. Beyond that, lower inter- and intra-assay variances could be measured for the analysis of Legionella pneumophila Serogroup 1, strain Bellingham-1. Even surface regeneration of indirect competitive immunoassays was possible, achieving a limit of detection of 0.35 ng L⁻¹ for enrofloxacin with polycarbonate microarray chips. Succinylated Jeffamine ED-2003 coated polycarbonate chips have great potential to replace microtiter plates by flow-based chemiluminescence microarrays for rapid analysis. Therefore, it helps analytical microarrays to advance into routine analysis and diagnostics.

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分析性微阵列具有强大的功能，可在一次测量中同时检测和定量多种分析物。在这项工作中，我们提出了一种快速简单的方法，用于在聚碳酸酯片材上批量制备微阵列。将琥珀酰化的Jeffamine®ED-2003丝网印刷在聚碳酸酯板上，通过在100°C下烘烤形成多功能屏蔽层。在以微阵列形式微分散捕获探针（抗体，寡核苷酸或小分子）后，将芯片与流动池由双面胶带组装在一起。结果表明，屏蔽层被牢固地覆盖并抑制了蛋白质的非特异性结合。通过将已建立的基于流的化学发光微阵列测量原理从载玻片转移到聚碳酸酯芯片，证明了通用性，而不会损失分析性能。通过在聚碳酸酯芯片上进行异质不对称重组酶聚合酶扩增，可以产生更高的化学发光信号。对于夹心微阵列免疫测定法可以显示相似的结果。除此之外，可以测量较低的测定间和测定内方差用于分析嗜肺军团菌血清群1，菌株Bellingham-1。间接竞争性免疫测定甚至可以进行表面再生，从而用聚碳酸酯微阵列芯片将恩诺沙星的检出限达到0.35 ng L ^-1。琥珀酰化的Jeffamine ED-2003涂层聚碳酸酯芯片具有巨大潜力，可通过基于流动的化学发光微阵列取代微量滴定板进行快速分析。因此，它有助于分析微阵列进入常规分析和诊断。

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