当前位置: X-MOL 学术Anal. Bioanal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Simultaneous detection of fumonisin B1 and ochratoxin A using dual-color, time-resolved luminescent nanoparticles (NaYF4: Ce, Tb and NH2-Eu/DPA@SiO2) as labels
Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2019-02-09 , DOI: 10.1007/s00216-019-01580-0
Sobia Niazi , Imran Mahmood Khan , Lv Yan , Muhammad Issa Khan , Ali Mohsin , Nuo Duan , Shijia Wu , Zhouping Wang

A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B1 (FB1) was developed using multi-color, Ln3+-doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF4: Ce, Tb and NH2-Eu/DPA@SiO2 NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized Fe3O4 magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb3+ and Eu3+, respectively, were used to measure FB1 (Y = 19,177.1 + (− 12,054.4)x, R2 = 0.9917) and OTA (Y = 4138.8 + (− 11,182.6)x, R2 = 0.9924), respectively. The limits of detection (LODs) for FB1 and OTA were 0.019 pg mL−1 and 0.015 pg mL−1, respectively, which were much lower than previously described methods for simultaneous recognition of mycotoxins OTA and FB1 while detection range varied from 0.0001–0.5 ng mL−1. This aptasensor was effectively applied to quantity FB1 and OTA in maize samples and results were compared with ELISA method. This is the first reported time-resolved fluorescence (TRF)-based aptasensor to detect two agriculturally important toxins in the maize. The developed aptasensor has potential to be used for detection of toxins in food safety fields.

Open image in new window

Graphical abstract



中文翻译:

同时检测伏马毒素B1个 和using曲霉毒素A使用双色,时间分辨的发光纳米粒子(NaYF4:Ce,Tb和NH2个-Eu / DPA @ SiO2个)作为标签

使用多色,Ln 3+掺杂的时间分辨荧光纳米粒子,开发了一种快速,高度灵敏的基于时间分辨荧光(TRF)的适体传感器,用于同时识别霉菌毒素och曲霉毒素A(OTA)和伏马菌素B 1(FB 1)。(TRF-NPs)(NaYF 4:Ce,Tb和NH 2 -Eu / DPA @ SiO 2 NPs)与互补链DNA(cDNA)耦合作为发光探针和适体偶联的胺官能化的Fe 3 O 4磁性纳米颗粒(MNPs )充当捕获探针。在最佳条件下,时间分辨荧光强度在544和618 nm对应于Tb 3+和Eu 3+分别用于测量FB 1Y  = 19,177.1 +(− 12,054.4)xR 2  = 0.9917)和OTA(Y  = 4138.8 +(− 11,182.6)xR 2  = 0.9924)。FB 1和OTA的检出限(LOD)分别为0.019 pg mL -1和0.015 pg mL -1,这比先前描述的同时识别霉菌毒素OTA和FB 1的方法低得多,而检测范围从0.0001改变–0.5 ng mL -1。该适体传感器已有效地应用于数量FB 1玉米样品中的OTA和OTA,并将结果与​​ELISA法进行比较。这是第一个报道的基于时间分辨荧光(TRF)的适体传感器,用于检测玉米中两种农业上重要的毒素。研发的适体传感器有潜力用于食品安全领域中的毒素检测。

在新窗口中打开图像

图形概要

更新日期:2019-02-09
down
wechat
bug