A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B₁ (FB₁) was developed using multi-color, Ln³⁺-doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF₄: Ce, Tb and NH₂-Eu/DPA@SiO₂ NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized Fe₃O₄ magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb³⁺ and Eu³⁺, respectively, were used to measure FB₁ (Y = 19,177.1 + (− 12,054.4)x, R² = 0.9917) and OTA (Y = 4138.8 + (− 11,182.6)x, R² = 0.9924), respectively. The limits of detection (LODs) for FB₁ and OTA were 0.019 pg mL⁻¹ and 0.015 pg mL⁻¹, respectively, which were much lower than previously described methods for simultaneous recognition of mycotoxins OTA and FB₁ while detection range varied from 0.0001–0.5 ng mL⁻¹. This aptasensor was effectively applied to quantity FB₁ and OTA in maize samples and results were compared with ELISA method. This is the first reported time-resolved fluorescence (TRF)-based aptasensor to detect two agriculturally important toxins in the maize. The developed aptasensor has potential to be used for detection of toxins in food safety fields.

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使用多色，Ln ³⁺掺杂的时间分辨荧光纳米粒子，开发了一种快速，高度灵敏的基于时间分辨荧光（TRF）的适体传感器，用于同时识别霉菌毒素och曲霉毒素A（OTA）和伏马菌素B ₁（FB ₁）。（TRF-NPs）（NaYF ₄：Ce，Tb和NH ₂ -Eu / DPA @ SiO ₂ NPs）与互补链DNA（cDNA）耦合作为发光探针和适体偶联的胺官能化的Fe ₃ O ₄磁性纳米颗粒（MNPs ）充当捕获探针。在最佳条件下，时间分辨荧光强度在544和618 nm对应于Tb ³⁺和Eu ³⁺分别用于测量FB ₁（Y  = 19,177.1 +（− 12,054.4）x，R ²  = 0.9917）和OTA（Y  = 4138.8 +（− 11,182.6）x，R ²  = 0.9924）。FB ₁和OTA的检出限（LOD）分别为0.019 pg mL ^-1和0.015 pg mL ^-1，这比先前描述的同时识别霉菌毒素OTA和FB _1的方法低得多，而检测范围从0.0001改变–0.5 ng mL ^-1。该适体传感器已有效地应用于数量FB ₁玉米样品中的OTA和OTA，并将结果与​​ELISA法进行比较。这是第一个报道的基于时间分辨荧光（TRF）的适体传感器，用于检测玉米中两种农业上重要的毒素。研发的适体传感器有潜力用于食品安全领域中的毒素检测。

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