Quantitative Proteomics Reveals Changes in Vero Cells in Response to Porcine Epidemic Diarrhea Virus.,Journal of Proteome Research

当前位置： X-MOL 学术 › J. Proteome Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Quantitative Proteomics Reveals Changes in Vero Cells in Response to Porcine Epidemic Diarrhea Virus.
Journal of Proteome Research ( IF 3.8 ) Pub Date : 2019-02-18 , DOI: 10.1021/acs.jproteome.8b00897
Yu Ye _{1,

2} , Jun Zhu _{1,

3} , Qiangyun Ai _{1,

4,

5} , Chengcheng Wang _{1,

4,

5} , Ming Liao _{1,

3,

4,

5} , Huiying Fan _{1,

3,

4,

5}

Affiliation

Outbreaks of porcine epidemic diarrhea virus (PEDV) have caused significant lethality rates in neonatal piglets, which pose a serious threat to the swine industry worldwide. Available commercial vaccines fail to protect against the emergence of high virulence of PEDV variants. Therefore, the endemic state of the PEDV infection in suckling piglets highlights the urgent need for uncovering the molecular determinants of the disease pathogenesis. In this study, stable isotope labeling by amino acids in cell culture (SILAC), combined with high-performance liquid chromatography/tandem mass spectrometry was performed to determine proteomic differences between PEDV-infected and mock-infected Vero cells at 18 h postinfection. The SILAC-based approach identified 4508 host-cell proteins, of which 120 were significantly up-regulated and 103 were significantly down-regulated at ≥95% confidence. Alterations in the expression of selected proteins were verified by Western blot. Several signaling metabolic pathways including mevalonate pathway I and the superpathway of cholesterol biosynthesis were triggered by the infection of the highly virulent strain and are linked to host innate immunity. 25-HC, an inhibitor of the mevalonate pathway, exhibited potent antiviral activity against PEDV infection. Meanwhile, the cell-cycle-related functions were significantly regulated, which may likely be responsible for the viral replication and pathogenicity of PEDV.

中文翻译：

定量蛋白质组学揭示了针对猪流行性腹泻病毒的Vero细胞的变化。

猪流行性腹泻病毒（PEDV）的爆发已导致新生仔猪的致死率很高，这对全世界的养猪业构成了严重威胁。可用的商业疫苗无法防止PEDV变异株出现高毒力。因此，乳猪中PEDV感染的流行状态凸显了迫切需要揭示疾病发病机理的分子决定因素。在这项研究中，进行了细胞培养物中氨基酸的稳定同位素标记（SILAC），并结合高效液相色谱/串联质谱法确定了感染后18 h PEDV感染和模拟感染的Vero细胞之间的蛋白质组学差异。基于SILAC的方法鉴定了4508个宿主细胞蛋白，置信度≥95％时，其中120个显着上调，而103个显着下调。通过蛋白质印迹证实所选蛋白质表达的改变。几种信号代谢途径包括甲羟戊酸途径I和胆固醇生物合成的超途径，是由高毒力菌株的感染触发的，并与宿主固有免疫力有关。甲羟戊酸途径的抑制剂25-HC对PEDV感染表现出有效的抗病毒活性。同时，细胞周期相关功能受到明显调节，这可能与PEDV的病毒复制和致病性有关。几种信号代谢途径包括甲羟戊酸途径I和胆固醇生物合成的超途径，是由高毒力菌株的感染触发的，并与宿主固有免疫力有关。甲羟戊酸途径的抑制剂25-HC对PEDV感染表现出有效的抗病毒活性。同时，细胞周期相关功能受到明显调节，这可能与PEDV的病毒复制和致病性有关。几种信号代谢途径包括甲羟戊酸途径I和胆固醇生物合成的超途径，是由高毒力菌株的感染触发的，并与宿主固有免疫力有关。甲羟戊酸途径的抑制剂25-HC对PEDV感染表现出有效的抗病毒活性。同时，细胞周期相关功能受到明显调节，这可能与PEDV的病毒复制和致病性有关。

更新日期：2019-02-19

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11