Herein, we developed a sensitive and quantitative flow assay for simultaneous detection of amantadine (AMD) and chloramphenicol (CAP) in chicken samples based on different CdSe/ZnS quantum dots (QDs). In contrast to other reports, the QDs could be excited by the same excitations that lowered the requirements for the matching instruments. Under the optimal conditions, the strategy permitted sensitive detection of AMD and CAP in a linear range of 0.23 to 1.02 ng/g and 0.02 to 0.66 ng/g. The limits of detection were 0.18 ng/g and 0.016 ng/g, respectively. Moreover, the whole detection process could be completed within 20 min with no additional sophisticated instruments and complicated operations. Spiked samples were analyzed using both QD-based lateral flow immunoassay (QD-LFIA) and commercial ELISA kits with good correlation (R² = 0.96). Moreover, this study laid the foundation and simplified the development of the requisite instrument.

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本文中，我们基于不同的CdSe / ZnS量子点（QD），开发了一种灵敏的定量流动分析法，可同时检测鸡肉样品中的金刚烷胺（AMD）和氯霉素（CAP）。与其他报告相反，可以通过降低匹配仪器要求的相同激励来激发QD。在最佳条件下，该策略允许在0.23至1.02 ng / g和0.02至0.66 ng / g的线性范围内灵敏地检测AMD和CAP。检测限分别为0.18 ng / g和0.016 ng / g。而且，整个检测过程可以在20分钟内完成，而无需其他复杂的仪器和复杂的操作。使用基于QD的侧向流免疫分析（QD-LFIA）和商业ELISA试剂盒对加标样品进行分析，相关性良好（R ²  ＝ 0.96）。此外，这项研究奠定了基础，并简化了必要工具的开发。

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