The aim of this study was to determine the typical incidence of micronuclei (MNi) in the peripheral blood lymphocytes of healthy middle-aged Koreans using the cytokinesis-block micronucleus cytome (CBMN-Cyt) assay. Non-smoking, low-risk alcohol-drinking healthy Korean men and women aged 30 to 59 years were recruited. Participants were divided into three groups according to age, i.e., 30 to 39, 40 to 49, and 50 to 59 years. Fifty participants were included in each age group, for a total of 300 participants. DNA damage was measured based on the number of binucleated (BN) cells with MNi, nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs) using the CBMN-Cyt assay. The frequencies of BN cells with MNi in men were 14.0+/-4.9 (mean+/-SD) in 30-39year olds, 20.0+/-6.1 in 40-49year olds, and 21.7+/-7.6 in 50-59year olds. In women, they were 19.7+/-7.1 in 30-39year olds, 28.7+/-11.2 in 40-49year olds, and 31.9+/-12.9 in 50-59year olds. MNi and NPBs scores were higher in females than in males. The elder groups showed higher MNi frequencies for both genders, and the NPB frequency was higher in elder groups than younger groups, but only for males. Based on a regression analysis of the CBMN-Cyt parameters, MNi frequencies showed a positive relationship with age for both genders. BMI and blood vitamin B concentration were not significantly associated with CBMN-Cyt parameters, except vitamin B6 levels, which were positively associated with MNi scores in males. These results provide the standard frequencies of MNi, NPBs, and NBUDs in peripheral blood lymphocytes in middle-aged Korean individuals with healthy lifestyles. In this group, CBMN-Cyt assay parameters varied according to gender and age; however, BMI and micronutrient levels were not significantly associated with assay parameters.

中文翻译：

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在健康的韩国中年人群中通过胞质分裂阻断微核细胞组测定评估的基因组健康状况。

本研究的目的是使用胞质分裂阻断微核细胞组 (CBMN-Cyt) 测定法确定健康中年韩国人外周血淋巴细胞中微核 (MNi) 的典型发生率。招募了 30 至 59 岁不吸烟、低风险饮酒的健康韩国男性和女性。参与者根据年龄分为三组，即30至39岁、40至49岁和50至59岁。每个年龄组包括 50 名参与者，总共 300 名参与者。使用 CBMN-Cyt 测定法根据具有 MNi、核质桥 (NPB) 和核芽 (NBUD) 的双核 (BN) 细胞的数量测量 DNA 损伤。男性中 BN 细胞与 MNi 的频率分别为 30-39 岁为 14.0+/-4.9（平均值+/-SD），40-49 岁为 20.0+/-6.1，50-59 岁为 21.7+/-7.6。在女性中，他们是 19.7+/-7。30-39 岁为 1，40-49 岁为 28.7+/-11.2，50-59 岁为 31.9+/-12.9。女性的 MNi 和 NPBs 得分高于男性。老年组男女均表现出较高的 MNi 频率，老年组的 NPB 频率高于年轻组，但仅限于男性。基于对 CBMN-Cyt 参数的回归分析，MNi 频率与男女的年龄呈正相关。除了维生素 B6 水平与男性的 MNi 评分呈正相关外，BMI 和血液维生素 B 浓度与 CBMN-Cyt 参数没有显着相关性。这些结果提供了健康生活方式的韩国中年人外周血淋巴细胞中 MNi、NPB 和 NBUD 的标准频率。在本组中，CBMN-Cyt 检测参数因性别和年龄而异；